BD 1 Rat

Beta Defensin-1 is characterized by its six-cysteine motif, which forms three intra-molecular disulfide bonds. This structure is essential for its stability and function. The recombinant form of Rat Beta Defensin-1 is typically produced in E. coli and consists of a single non-glycosylated polypeptide chain containing 37 amino acids, with a molecular weight of approximately 4.1 kDa .

Beta Defensin-1 exhibits potent antimicrobial properties. It interacts with the negatively charged membranes of invading microbes, leading to membrane destabilization and microbial death. Additionally, Beta Defensin-1 enhances the adaptive immune response by promoting the chemotaxis of immune cells such as monocytes, T-lymphocytes, dendritic cells, and mast cells to the site of infection .

Recombinant Rat Beta Defensin-1 is widely used in research to study its antimicrobial properties and its role in the immune system. It is also utilized in various bioassays to evaluate its biological activity, particularly in chemotaxis assays using CD34+ dendritic cells .

The recombinant protein is typically supplied as a lyophilized powder and should be reconstituted in sterile distilled water or an appropriate buffer. It is recommended to store the reconstituted protein in aliquots at -20°C to -80°C to avoid repeated freeze-thaw cycles, which can degrade the protein .