Bartonella SucB
Escherichia Coli.
Greater than 95% as determined by SDS-PAGE.
Description
Recombinant Bartonella Henselae SucB produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 48kDa.
Bartonella SucB is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Escherichia Coli.
Product Science Overview
Bartonella henselae is a gram-negative, facultative intracellular bacterium that is primarily transmitted to humans through cat scratches or bites, as well as via cat fleas (Ctenocephalides felis) . This bacterium is the causative agent of several diseases, including cat scratch disease (CSD), bacillary angiomatosis, and infective endocarditis (IE) . Bartonella henselae is of significant epidemiological and pathological concern due to its ability to cause a wide spectrum of diseases, particularly in immunocompromised individuals .
The SucB protein is a component of the 2-oxoglutarate dehydrogenase complex, which plays a crucial role in the citric acid cycle (Krebs cycle). This enzyme complex is involved in the oxidative decarboxylation of 2-oxoglutarate to succinyl-CoA and CO2, a key step in cellular respiration and energy production . In Bartonella henselae, the SucB protein is essential for the bacterium’s metabolic processes and survival within host cells.
Recombinant proteins are proteins that are genetically engineered in the laboratory by inserting the gene encoding the protein of interest into an expression system, such as bacteria, yeast, or mammalian cells. The recombinant SucB protein from Bartonella henselae is produced by cloning the sucB gene into an appropriate expression vector, which is then introduced into a host organism for protein production .
The production of recombinant SucB protein involves several steps:
- Gene Cloning: The sucB gene is amplified using polymerase chain reaction (PCR) and inserted into an expression vector.
- Transformation: The expression vector is introduced into a host organism, such as Escherichia coli, through a process called transformation.
- Protein Expression: The host organism is cultured under conditions that induce the expression of the recombinant SucB protein.
- Protein Purification: The recombinant SucB protein is purified from the host cells using techniques such as affinity chromatography .
The recombinant SucB protein from Bartonella henselae has several important applications in research and diagnostics:
- Serodiagnosis: Recombinant SucB protein can be used as an antigen in serological assays, such as enzyme-linked immunosorbent assays (ELISA), to detect antibodies against Bartonella henselae in patient sera .
- Vaccine Development: The recombinant protein can be explored as a potential vaccine candidate to prevent Bartonella henselae infections .
- Functional Studies: Researchers can use the recombinant SucB protein to study the biochemical properties and functions of the protein, as well as its role in the pathogenesis of Bartonella henselae .
