Bartonella 26kDa
Escherichia Coli.
Greater than 95% as determined by SDS-PAGE.
Description
Recombinant Bartonella Henselae 26kDa produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 25kDa.
Bartonella 26kDa is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Escherichia Coli.
Product Science Overview
Bartonella henselae is a gram-negative, facultative intracellular bacterium that is primarily known for causing cat scratch disease (CSD) in humans. This pathogen is transmitted through cat scratches or bites and is also associated with other severe conditions, particularly in immunocompromised individuals. The 26kDa recombinant protein of Bartonella henselae has garnered significant interest due to its potential applications in diagnostics and research.
Bartonella henselae is a member of the Alphaproteobacteria subdivision and is capable of establishing an intracellular lifestyle within eukaryotic cells. In immunocompetent humans, infection typically results in CSD, characterized by regional lymphadenopathy. However, in immunosuppressed individuals, the infection can lead to severe conditions such as bacillary angiomatosis, peliosis hepatis, endocarditis, and bacteremia .
The 26kDa protein, also referred to as p26, is an immunodominant antigen expressed by Bartonella henselae. This protein has been identified as a potential marker for molecular diagnosis and species-level identification of Bartonella infections . The gene encoding the 26kDa protein shows significant nucleotide identity with orthologs in other bacteria such as Brucella and several plant-associated bacteria .
The gene encoding the 26kDa protein has been successfully cloned and expressed in Escherichia coli. The recombinant protein is produced as a single, non-glycosylated polypeptide chain with a molecular mass of approximately 25kDa . The expression of this protein in E. coli has facilitated its purification and subsequent use in various immunological assays.
The 26kDa protein is highly immunoreactive and has been shown to elicit strong immune responses in infected hosts. Studies have demonstrated that feline antisera raised against Bartonella henselae react with the recombinant 26kDa protein, indicating its potential as a diagnostic antigen . Additionally, murine hyperimmune serum raised against the recombinant protein also reacts with the protein, further supporting its immunogenicity .
The immunoreactivity of the 26kDa protein makes it a valuable tool for the serodiagnosis of Bartonella henselae infections. Immunoproteomic approaches have identified this protein as a candidate for differentiating between various clinical manifestations of bartonellosis, such as CSD and infective endocarditis . The use of the 26kDa recombinant protein in enzyme-linked immunosorbent assays (ELISA) and other immunoassays can enhance the accuracy and specificity of diagnostic tests for Bartonella infections.
