Bartonella 17kDa
Escherichia Coli.
Greater than 95% as determined by SDS-PAGE.
Description
Recombinant Bartonella Henselae 17kDa produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 18kDa.
Bartonella 17kDa is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Bartonellosis encompasses various forms and is primarily caused by the bacterium Bartonella henselae. This bacterium is responsible for diseases like Cat Scratch Disease (CSD) and Bacillary Angiomatosis. Studies indicate that a significant proportion of CSD patients, up to 95%, exhibit antibodies targeting Bartonella henselae antigens. Among these antigens, P17 stands out as the first discovered antigen demonstrating strong reactivity with sera from CSD patients. Notably, Bartonella henselae utilizes a type IV secretion system, with P17 acting as a homolog of the virB5 family, to facilitate host recognition. The diagnosis of CSD heavily relies on highly immunoreactive proteins produced by Bartonella henselae, which serve as crucial antigens.
Recombinant Bartonella Henselae 17kDa, produced using E. coli as the expression system, is a single, non-glycosylated polypeptide chain with an approximate molecular mass of 18kDa. It features a -10x His tag positioned at the N-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
Bartonella 17kDa is provided in a solution containing 20mM HEPES buffer at pH 8.0 and 6M Urea.
For short-term storage (2-4 weeks), maintain the product at a temperature of 4 degrees Celsius. For extended storage, store the product in a frozen state at -20 degrees Celsius. To preserve product integrity and performance, it is recommended to minimize repeated freeze-thaw cycles.
The purity of the product is determined using SDS-PAGE analysis and exceeds 95%.
Escherichia Coli.
Product Science Overview
Bartonella henselae is a gram-negative, facultative intracellular bacterium that is primarily transmitted to humans through cat scratches or bites, as well as via cat fleas (Ctenocephalides felis) . It is the causative agent of several diseases, including cat scratch disease (CSD), bacillary angiomatosis, and infective endocarditis (IE) . The bacterium has a unique invasion mechanism that drives angiogenesis both in vitro and in vivo .
The 17kDa protein of Bartonella henselae is one of the key antigens used in the serodiagnosis of bartonellosis. This protein is often produced recombinantly for research and diagnostic purposes. The recombinant form of the 17kDa protein is typically expressed in E. coli and purified using chromatographic techniques . It is a single, non-glycosylated polypeptide chain with a molecular mass of approximately 18kDa, including a 10x His tag at the N-terminus .
The 17kDa protein, along with other antigens such as GroEL, P26, BadA, Pap31, OMP 89, and OMP 43, has been identified as a significant marker for the diagnosis of Bartonella henselae infections . These proteins are used in various immunoproteomic approaches to differentiate between clinical scenarios such as CSD and IE . The use of recombinant proteins in diagnostic assays enhances the sensitivity and specificity of tests like enzyme-linked immunosorbent assays (ELISA) and immunofluorescent assays (IFA) .
Molecular methods, including PCR and real-time PCR, are employed to detect Bartonella henselae DNA in clinical samples . These methods are crucial for confirming infections, especially in cases where serological tests may yield false-negative results due to the bacterium’s low bacteremia and fastidious nature . The combination of molecular techniques with recombinant protein-based assays provides a comprehensive approach to diagnosing bartonellosis.
