B2M Human Recombinant

Beta 2 Microglobulin Human Recombinant
Cat. No.
BT26442
Source
Escherichia Coli.
Synonyms
Beta-2-microglobulin, B2M.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

B2 Microglobulin Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain having a molecular mass of 11.76 kDa.
The B2M is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Beta-2 microglobulin (β2M) is an 11 kDa protein found on the surface of many cells, including lymphocytes, and serves as the small subunit of the MHC class I molecule. β2M association is crucial for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Besides MHC class I molecules, β2M also interacts with class I-like molecules like CD1 and Qa. The absence of β2M significantly limits the presence of MHC class I molecules on the cell surface, impacting CD8 T cell development. β2M is found in low concentrations in the serum, cerebrospinal fluid, and urine of healthy individuals. However, significantly elevated levels are observed in individuals with conditions such as tubular proteinuria, renal failure, or kidney transplants. Increased β2M levels can indicate increased synthesis (e.g., in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumors, and autoimmune diseases) or impaired renal filtration (e.g., due to renal insufficiency, graft rejection, or nephrotoxicity from post-transplantation immunosuppressive therapy). Elevated β2M levels may also be present in multiple myeloma and lymphoma cases. Long-term hemodialysis can lead to dialysis-related amyloidosis, where β2M aggregates into amyloid fibers and deposits in joint spaces.
Description
Recombinant Human B2 Microglobulin, produced in E. coli, is a non-glycosylated polypeptide chain with a molecular weight of 11.76 kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a 1 mg/ml solution containing PBS (pH 7.4) and 0.05% NaN3.
Solubility
To reconstitute the lyophilized B2M, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration not less than 100 µg/ml. The reconstituted solution can be further diluted in other aqueous solutions.
Stability
Lyophilized B2M is stable at room temperature for up to 3 weeks; however, it is recommended to store the desiccated product below -18°C. After reconstitution, B2M should be stored at 4°C for 2-7 days. For long-term storage, store below -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Beta-2-microglobulin, B2M.
Source
Escherichia Coli.

Product Science Overview

Structure and Function

B2M is a small protein consisting of 119 amino acids. It is non-covalently associated with the alpha chains of MHC class I molecules. This association is critical for the stability and function of the MHC class I complex. The MHC class I molecules display fragments of proteins from within the cell on the cell surface. This allows T-cells to monitor the health of cells and detect any that are infected with viruses or have become cancerous .

Recombinant Production

Recombinant human B2M is produced using various expression systems, including E. coli and HEK293 cells. The recombinant protein is often tagged with a polyhistidine (His) tag to facilitate purification. After expression, the protein is purified to high levels of purity, typically greater than 95%, as determined by methods such as SDS-PAGE and HPLC .

Clinical Significance

B2M levels in the blood and urine can be used as a biomarker for various diseases. Elevated levels of B2M are associated with certain cancers, including multiple myeloma and lymphoma, as well as kidney diseases. It is also used as a marker for monitoring the progression of these diseases and the effectiveness of treatments .

Applications

Recombinant B2M is used in various research and clinical applications. It is utilized in studies related to the immune system, cancer research, and the development of diagnostic assays. The protein’s role in antigen presentation makes it a valuable tool for understanding immune responses and developing new therapeutic strategies .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

B2M Antibody
Beta 2 Microglobulin, Mouse Anti Human
Cat. No.
BT12798
Source
B2M Human
Beta-2 Microglobulin Human
Cat. No.
BT26353
Source
Human urine from patients with tubular proteinuria.
B2M Human, His
Beta 2 Microglobulin Human Recombinant, His Tag
Cat. No.
BT26513
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.