B2M Antibody
Description
Product Specs
Product Science Overview
Beta 2 Microglobulin (β2M) is a small protein that is a component of the major histocompatibility complex (MHC) class I molecules, which are present on the surface of nearly all nucleated cells. It plays a crucial role in the immune system by presenting peptide antigens to T cells. The mouse anti-human β2M antibody is a monoclonal antibody that specifically targets human β2M, making it a valuable tool in various scientific and medical applications.
β2M is a 12 kDa non-polymorphic protein that is non-covalently associated with the heavy chains of MHC class I molecules. This association is essential for the stability and function of MHC class I molecules, which are involved in the presentation of endogenous antigens to CD8+ T cells. The β2M protein itself is composed of a single polypeptide chain and has a structure similar to that of immunoglobulin domains .
Elevated levels of β2M in the blood can be indicative of various medical conditions, including chronic kidney disease, certain cancers, and autoimmune disorders. It is often used as a biomarker to assess renal function and to monitor the progression of diseases such as multiple myeloma and lymphoma .
The mouse anti-human β2M antibody is a monoclonal antibody that is produced by immunizing mice with human β2M. This antibody is highly specific to human β2M and does not cross-react with β2M from other species. It is widely used in research and clinical diagnostics due to its ability to detect β2M in various applications, including Western blotting, immunohistochemistry, flow cytometry, and ELISA .
- Western Blotting: The mouse anti-human β2M antibody is used to detect β2M in protein samples separated by gel electrophoresis. This technique is commonly used to study the expression levels of β2M in different tissues and under various experimental conditions.
- Immunohistochemistry: This antibody is used to stain tissue sections to visualize the distribution and localization of β2M in different cell types. It is particularly useful in studying the role of β2M in immune responses and in the pathology of diseases.
- Flow Cytometry: The antibody is used to label cells for flow cytometric analysis, allowing researchers to quantify the expression of β2M on the surface of cells. This application is important for studying immune cell populations and their activation states.
- ELISA: The mouse anti-human β2M antibody is used in enzyme-linked immunosorbent assays to measure the concentration of β2M in biological samples. This is a common method for monitoring β2M levels in clinical diagnostics .
