ATP1B2 Human, Sf9
Description
Product Specs
Product Science Overview
The Na+/K+ ATPase enzyme is composed of two main subunits: a large catalytic alpha subunit and a smaller glycoprotein beta subunit. The beta subunit, including ATP1B2, plays a regulatory role by assembling alpha/beta heterodimers, which determine the number of sodium pumps transported to the plasma membrane . The beta-2 subunit specifically mediates cell adhesion of neurons and astrocytes and promotes neurite outgrowth .
The primary function of ATP1B2 is to catalyze the hydrolysis of ATP, coupled with the exchange of Na+ and K+ ions across the plasma membrane. This process is vital for maintaining the resting potential, affecting transport, and regulating cellular volume . Although the exact function of the beta-2 subunit is not fully understood, it is known to play a role in cell adhesion and neurite outgrowth .
ATP1B2 is involved in various physiological processes. It helps maintain the sodium and potassium gradients across the plasma membrane, which are used by animal cells for numerous processes, including secondary transport of molecules and rapid signaling . The sodium gradient is particularly crucial in organs like the kidneys, where it is utilized for filtering blood, reabsorbing glucose and amino acids, and regulating electrolytes and pH .
Recombinant ATPase Transporting Beta 2 is often produced in Sf9 cells, a type of insect cell line derived from the fall armyworm. This system is widely used for the production of recombinant proteins due to its high expression levels and ability to perform post-translational modifications similar to those in mammalian cells .
Recombinant ATP1B2 has various applications in research and medicine. It is used to study the structure and function of the Na+/K+ ATPase enzyme, investigate its role in different physiological processes, and develop potential therapeutic interventions for diseases related to ion transport dysfunction .
