ARL3 Human
ADP-Ribosylation Factor-Like 3 Human Recombinant
Cat. No.
BT14132
Source
Escherichia Coli.
Synonyms
ADP-ribosylation factor-like protein 3, ARL3, ARFL3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ARL3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 206 amino acids (1-182 a.a.) and having a molecular mass of 23kDa.
ARL3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
ARL3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
ARL3 (ARFL3) is a member of the ARF-like protein (ARL) subfamily, which are small GTPases. Unlike ARFs, ARL3 does not activate cholera toxin ADP-ribosyltranferase. It is ubiquitously expressed and may play a role in maintaining rod photoreceptor cells and regulating microtubule-dependent processes. Studies have shown that ARL3 knockdown can result in abnormal cell morphology, cytokinesis failure, alpha-tubulin acetylation, and an increase in binucleated cells.
Description
Recombinant human ARL3 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It contains 206 amino acids (including a 24 amino acid His-tag at the N-terminus) and has a molecular weight of 23kDa. The protein sequence comprises amino acids 1-182 of the ARL3 protein, with an additional His-tag for purification. ARL3 is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ARL3 protein is supplied in a solution at a concentration of 1mg/ml. The solution contains 20mM Tris-HCl buffer (pH 8.0), 30% glycerol, 0.1M NaCl, 1mM DTT, and 0.1mM PMSF.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freezing and thawing of the product.
Purity
The purity of ARL3 protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
ADP-ribosylation factor-like protein 3, ARL3, ARFL3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMGLLSI LRKLKSAPDQ EVRILLLGLD NAGKTTLLKQ LASEDISHIT PTQGFNIKSV QSQGFKLNVW DIGGQRKIRP YWKNYFENTD ILIYVIDSAD RKRFEETGQE LAELLEEEKL SCVPVLIFAN KQDLLTAAPA SEIAEGLNLH TIRDRVWQIQ SCSALTGEGV QDGMNWVCKN VNAKKK.
Product Science Overview
Structure and Function
ARL3, like other members of the ARF family, binds guanine nucleotides (GTP and GDP) but does not activate the cholera toxin ADP-ribosyltransferase, which differentiates it from other ARF proteins . The protein is ubiquitously expressed in various tissues and is involved in several cellular functions, including:
- Vesicular Trafficking: ARL3 plays a role in the transport of vesicles within cells, which is crucial for maintaining cellular homeostasis and function .
- Microtubule-Dependent Processes: It is involved in the regulation of processes that depend on microtubules, such as cell division and intracellular transport .
- Photoreceptor Cell Maintenance: ARL3 is believed to have a role in maintaining rod photoreceptor cells, which are essential for vision .
Genetic and Molecular Insights
Clinical Relevance
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