APOD Human, HEK
Apolipoprotein-D Human Recombinant, HEK
Cat. No.
BT11614
Source
HEK 293.
Synonyms
Apolipoprotein D, Apo-D, ApoD.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Apolipoprotein-D Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (aa 21-189) containing a total of 175 amino acids, having a molecular mass of 20.1kDa (calculated) and fused to a 6 aa His tag at C-Terminus.
The Human APOD is purified by proprietary chromatographic techniques.
The Human APOD is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Apolipoprotein-D, a member of the lipocalin family, primarily associates with high-density lipoproteins in human plasma. Unlike typical apolipoproteins, it displays a beta-barrel structure, facilitating the transportation of small hydrophobic ligands such as cholesterol, progesterone, pregnenolone, bilirubin, and arachidonic acid. Notably expressed in the spleen, testes, and brain, Apolipoprotein-D is found in high concentrations within the cyst fluid of individuals with gross cystic disease of the breast, a condition linked to an elevated risk of breast cancer. Its accumulation is also observed in regenerating peripheral nerves and the cerebrospinal fluid of patients with neurodegenerative disorders like Alzheimer's disease. Apolipoprotein-D plays a crucial role in the maintenance and repair processes of the central and peripheral nervous systems. Functioning as a versatile transporter, it facilitates ligand transfer between cells within an organ, scavenges ligands for transport to the bloodstream, and delivers ligands from circulation to specific tissues.
Description
Recombinantly produced in HEK cells, Apolipoprotein-D Human Recombinant is a single, glycosylated polypeptide chain encompassing amino acids 21-189. With a total of 175 amino acids, it possesses a molecular mass of 20.1kDa (calculated) and is fused to a 6 aa His tag at the C-terminus. The purification of Human APOD is achieved through proprietary chromatographic methods.
Physical Appearance
White lyophilized powder, filtered for optimal quality.
Formulation
The product undergoes filtration (0.4µm) and lyophilization from a solution of 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, maintaining a pH of 7.4.
Solubility
To prepare a working solution, add deionized water to achieve a concentration of 0.5mg/ml and ensure complete dissolution of the lyophilized pellet. Prior to cell culture use, filter the product through a sterile filter as it is not supplied sterile.
Stability
For long-term storage, keep the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freeze-thaw cycles. Reconstituted protein remains stable at 4°C for a limited duration.
Purity
Analysis by SDS-PAGE indicates a purity greater than 80.0%.
Synonyms
Apolipoprotein D, Apo-D, ApoD.
Source
HEK 293.
Amino Acid Sequence
QAFHLGKCPN PPVQENFDVN KYLGRWYEIE KIPTTFENGR CIQANYSLME NGKIKVLNQE LRADGTVNQI EGEATPVNLT EPAKLEVKFS WFMPSAPYWI LATDYENYAL VYSCTCIIQL FHVDFAWILA RNPNLPPETV DSLKNILTSN NIDVKKMTVT DQVNCPKLSH HHHHH.
Product Science Overview
Structure and Expression
Apolipoprotein-D is a glycoprotein with a molecular weight of approximately 20.1 kDa . It consists of 175 amino acids and is characterized by its ability to bind and transport a variety of ligands in different contexts . The recombinant form of Apo-D, expressed in Human Embryonic Kidney (HEK) 293 cells, is often used in research due to its high purity and biological activity .
Biological Functions
Apo-D is associated with several important biological functions:
- Lipid Transport: Apo-D is involved in the transport and binding of lipids, particularly cholesterol and phospholipids .
- Neurological Disorders: Elevated levels of Apo-D have been observed in patients with neurological disorders such as schizophrenia, bipolar disorder, and Alzheimer’s disease .
- Nerve Injury Response: Apo-D plays a role in the response to nerve injury, particularly related to the myelin sheath .
Clinical Significance
Apo-D has been identified as a potential biomarker for various conditions:
- Androgen Insensitivity Syndrome: Apo-D is considered a putative biomarker for androgen receptor function in androgen insensitivity syndrome, a common cause of disorders of sex development .
- Stroke: Elevated levels of Apo-D have been found in a rat model of stroke, indicating its potential role in stroke pathology .
Recombinant Apo-D in Research
The recombinant form of Apo-D, expressed in HEK 293 cells, is widely used in research for its stability and suitability for various applications, including mass spectrometry, SDS-PAGE, ELISA, and Western Blotting . The recombinant protein is often tagged with a polyhistidine tag to facilitate purification and detection .
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