ANXA1 Mouse

Annexin A1 Mouse Recombinant
Cat. No.
BT19802
Source
Escherichia Coli.
Synonyms
Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ANXA1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 369 amino acids (1-346 a.a) and having a molecular mass of 41.1kDa.
ANXA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ANXA1, a member of the Ca(2+)-dependent phospholipid binding protein family, is localized to the cytosolic face of the plasma membrane and has a molecular weight ranging from 35kDa to 40kDa. With a molecular weight of 40kDa, ANXA1 exhibits high-affinity binding to two to four calcium ions and possesses phospholipase A2 inhibitory activity. Given the role of phospholipase A2 in the biosynthesis of prostaglandins and leukotrienes, potent inflammatory mediators, ANXA1 is thought to have potential anti-inflammatory effects. Moreover, ANXA1 plays a crucial role in exocytosis by promoting membrane fusion. Immunocytochemical recognition of ANXA1 protein provides a straightforward, highly sensitive, and specific assay for diagnosing hairy cell leukemia.
Description
Recombinant ANXA1 Mouse, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 369 amino acids (1-346 a.a), has a molecular mass of 41.1 kDa, and is fused to a 23 amino acid His-tag at its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ANXA1 protein solution (1mg/ml) is supplied in a buffer consisting of phosphate buffered saline (pH 7.4), 20% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage periods. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 95.0% using SDS-PAGE analysis.
Synonyms
Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAMVSEF LKQARFLENQ EQEYVQAVKS YKGGPGSAVS PYPSFNVSSD VAALHKAIMV KGVDEATIID ILTKRTNAQR QQIKAAYLQE NGKPLDEVLR KALTGHLEEV VLAMLKTPAQ FDADELRGAM KGLGTDEDTL IEILTTRSNE QIREINRVYR EELKRDLAKD ITSDTSGDFR KALLALAKGD RCQDLSVNQD LADTDARALY EAGERRKGTD VNVFTTILTS RSFPHLRRVF QNYGKYSQHD MNKALDLELK GDIEKCLTTI VKCATSTPAF FAEKLYEAMK GAGTRHKALI RIMVSRSEID MNEIKVFYQK KYGISLCQAI LDETKGDYEK ILVALCGGN.

Product Science Overview

Biological Role and Functions

ANXA1 plays a crucial role in the regulation of inflammation and the immune response. It mediates some of the anti-inflammatory effects of glucocorticoids, which are hormones that modulate inflammation and immune responses . The protein is involved in various cellular processes, including:

  • Inhibition of Phospholipase A2: ANXA1 inhibits phospholipase A2, an enzyme that releases arachidonic acid from membrane phospholipids, leading to the production of pro-inflammatory eicosanoids .
  • Regulation of Leukocyte Migration: ANXA1 helps regulate the migration of leukocytes (white blood cells) to sites of inflammation, thereby controlling the extent of the inflammatory response .
  • Promotion of Apoptosis: ANXA1 promotes apoptosis (programmed cell death) in certain cell types, which is essential for the resolution of inflammation and the maintenance of tissue homeostasis .
Therapeutic Potential

Given its significant role in inflammation, ANXA1 has been extensively studied for its therapeutic potential in various inflammatory diseases. Research has shown that ANXA1 and its mimetics can effectively limit inflammation in experimental models of myocardial ischemia/reperfusion injury, arthritis, stroke, multiple sclerosis, and sepsis . The protein’s ability to promote the resolution of inflammation makes it a promising target for drug discovery.

Recombinant ANXA1

Recombinant ANXA1 (Mouse) is a form of the protein that is produced through recombinant DNA technology. This involves inserting the gene encoding ANXA1 into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. Recombinant ANXA1 is used in research to study its biological functions and therapeutic potential.

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THE BIOTEK
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