ANXA1 Antibody
Annexin A1, Mouse Anti Human
Cat. No.
BT12291
Source
Synonyms
ANX1,
LPC1, ANXA1, Lipocortin I, Calpactin II, Chrombindin-9, p35, Annexin-1,
Phospholipase A2 inhibitory protein, Annexin I, Annexin A1.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Product Specs
Introduction
ANXA1, a member of the Ca(2+)-dependent phospholipid binding protein family, plays a crucial role in regulating inflammation and membrane fusion. These proteins, with molecular weights ranging from 35kDa to 40kDa, reside on the cytoplasmic side of the plasma membrane. ANXA1, specifically, exhibits a molecular weight of 40kDa and possesses phospholipase A2 inhibitory activity, enabling it to bind two to four calcium ions with high affinity. This inhibition of phospholipase A2, an enzyme essential for the production of inflammatory mediators like prostaglandins and leukotrienes, suggests potential anti-inflammatory properties of ANXA1. Moreover, ANXA1 facilitates membrane fusion and participates in exocytosis. The ability to identify ANXA1 protein through immunocytochemistry provides a straightforward, highly sensitive, and specific method for diagnosing hairy cell leukemia.
Physical Appearance
A clear, colorless solution that has undergone sterile filtration.
Formulation
The solution is prepared at a concentration of 1mg/ml and contains the following components: PBS (pH 7.4), 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), the solution should be kept at 4°C. For extended storage periods, it is recommended to store at -20°C. To maintain stability, avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has undergone rigorous testing through ELISA and Western blot analysis, confirming its specificity and reactivity. However, it's essential to note that optimal results may vary depending on the specific application. Therefore, titration of the reagent is recommended for each individual investigation to determine the most effective working concentration.
Synonyms
ANX1,
LPC1, ANXA1, Lipocortin I, Calpactin II, Chrombindin-9, p35, Annexin-1,
Phospholipase A2 inhibitory protein, Annexin I, Annexin A1.
Purification Method
ANXA1 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT2G5AT
Immunogen
Anti-human ANXA1 mAb,
clone PAT2G5AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human ANXA1 protein 1-346
amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Annexin A1
Annexin A1, also known as lipocortin I, is a protein encoded by the ANXA1 gene in humans . It belongs to the annexin family of Ca²⁺-dependent phospholipid-binding proteins, which have a molecular weight of approximately 35,000 to 40,000 Daltons . These proteins are preferentially located on the cytosolic face of the plasma membrane .
Function and Significance:
- Anti-inflammatory Activity: Annexin A1 plays a crucial role in the anti-inflammatory response by inhibiting phospholipase A2, which blocks eicosanoid production . It also inhibits various leukocyte inflammatory events such as epithelial adhesion, emigration, chemotaxis, phagocytosis, and respiratory burst .
- Immune Response: Annexin A1 is involved in both innate and adaptive immunity. It promotes neutrophil detachment and apoptosis, and the phagocytosis of apoptotic neutrophils by macrophages . It also enhances signaling cascades triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells, and promotes the differentiation of T-cells into Th1 cells while negatively regulating differentiation into Th2 cells .
- Clinical Relevance: Annexin A1 has been implicated in various diseases, including cancer. It protects against DNA damage induced by heat in breast cancer cells, indicating its tumor suppressive and protective activities .
Mouse Anti-Human Antibodies
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins . These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins . They are widely used in various applications, including detection, sorting, and purification of human targets.
Applications:
- Detection Systems: Mouse anti-human antibodies are used in various detection systems such as HRP, AP, and fluorescence . They provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody .
- Research and Diagnostics: These antibodies are commonly used in research and diagnostic applications, including ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry .
HAMA Response:
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