Anaplasma p44
Escherichia Coli.
Greater than 95% as determined by SDS-PAGE.
Description
Recombinant Anaplasma p44 produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 49kDa.
Anaplasma p44 is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Escherichia Coli.
Product Science Overview
The p44 protein is the immunodominant and most abundant outer membrane protein of Anaplasma phagocytophilum . It belongs to the p44/msp2 multigene family, which includes more than 80 p44 paralogs dispersed throughout the genome . These proteins play a crucial role in the bacterium’s ability to evade the host’s immune system by varying their surface antigens, a phenomenon known as antigenic variation .
The recombinant p44 protein is produced using Escherichia coli as the expression system . The recombinant protein is typically fused to a his-tag at its N-terminal to facilitate purification . The molecular weight of the recombinant p44 protein, as determined by SDS-PAGE, is approximately 46,060.39 Da . The protein is presented in a liquid solution and is stored at -20°C to -80°C to maintain its stability .
The recombinant p44 protein is used in various diagnostic applications, including Western Blot, Dot Blot, Indirect ELISA, and Chemiluminescent Immunoassay (CLIA) . It serves as a critical tool for the diagnosis of anaplasmosis in both humans and animals, including dogs, cats, horses, sheep, and cattle . The 44-kDa-protein-specific antibodies play a significant role in immunity against infection, making the recombinant p44 protein an essential component in understanding the pathogenesis and immune response to Anaplasma phagocytophilum .
