Anaplasma Msp5
Sf9 insect cells.
Major surface protein 5, msp5.
Greater than 80% as determined by SDS-PAGE.
Description
Recombinant Anaplasma Msp5 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 20kDa.
Anaplasma Msp5 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Major surface protein 5, msp5.
Sf9 insect cells.
Product Science Overview
Major Surface Protein 5 (Msp5) is one of the key proteins found on the surface of Anaplasma phagocytophilum. This protein is highly conserved across different species within the genus Anaplasma, making it a crucial target for diagnostic and research purposes . The conservation of Msp5 allows for the development of diagnostic tools that can detect antibodies against Anaplasma spp. in infected hosts.
Recombinant Msp5 (rMsp5) refers to the laboratory-produced version of the Msp5 protein. By using recombinant DNA technology, scientists can produce large quantities of Msp5 in a controlled environment, which is essential for research and diagnostic applications. The recombinant form of Msp5 is used in various assays, such as enzyme-linked immunosorbent assays (ELISAs), to detect the presence of antibodies against Anaplasma spp. in serum samples .
- Diagnostic Tool: The recombinant Msp5 protein is widely used in competitive ELISAs (cELISAs) to detect antibodies against Anaplasma spp. in infected hosts. This is particularly useful in veterinary medicine for diagnosing anaplasmosis in cattle .
- Cross-Reactivity Studies: Studies have shown that rMsp5 of Anaplasma phagocytophilum exhibits cross-reactivity with Anaplasma marginale, another species within the genus. This cross-reactivity is significant for understanding the serologic relationships between different Anaplasma species and improving diagnostic accuracy .
- Research: The availability of recombinant Msp5 allows researchers to study the protein’s structure, function, and interactions with the host immune system. This knowledge can contribute to the development of new therapeutic strategies and vaccines against anaplasmosis.
