ALPL Mouse
Alkaline Phosphatase Liver/Bone/Kidney Mouse Recombinant
Cat. No.
BT27115
Source
Sf9, Baculovirus cells.
Synonyms
Alpl,
Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS, AP-TNAP, Alkaline phosphatase 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
ALPL produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 493 amino acids (19-503 a.a.) and
having a molecular mass of 54.5kDa (Molecular size on SDS-PAGE will appear at
approximately 50-70kDa). ALPL is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Alkaline Phosphatase Liver/Bone/Kidney (Alpl), a member of the alkaline phosphatases family, encompasses four related alkaline phosphatases. This membrane-bound and glycosylated enzyme lacks tissue-specific expression and plays a crucial role in skeletal mineralization.
Description
Produced in Sf9 Baculovirus cells, ALPL is a single, glycosylated polypeptide chain with 493 amino acids (19-503 a.a.) and a molecular mass of 54.5kDa. Note that its size on SDS-PAGE appears between 50-70kDa. The protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
The ALPL protein solution (0.5mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Minimize repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity, exceeding 46,000 pmol/min/ug, represents the enzyme's ability to hydrolyze 1 picomole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 and 25°C.
Synonyms
Alpl,
Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS, AP-TNAP, Alkaline phosphatase 2.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT
AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR
CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL
MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLAL
DPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQA
LHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNG
PGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYAS
CIGANLDHCA WAGSGLEHHH HHH.
Product Science Overview
Classification and Isoforms
Alkaline phosphatase exists in multiple isoforms, each encoded by different genes and exhibiting tissue-specific expression. In humans, there are four distinct genes encoding alkaline phosphatases:
Biological Properties and Functions
The liver/bone/kidney isozyme of alkaline phosphatase, encoded by the ALPL gene, is highly expressed in these tissues and plays a vital role in various biological processes:
Recombinant Alkaline Phosphatase
Recombinant alkaline phosphatase is produced using genetic engineering techniques, where the gene encoding the enzyme is inserted into a host organism, such as bacteria or yeast, to produce the enzyme in large quantities. This recombinant form is used in research and clinical applications due to its high purity and consistency.
Applications in Research and Medicine
Recombinant alkaline phosphatase is widely used in various research and medical applications:
- Diagnostic Marker: Elevated levels of alkaline phosphatase in the blood can indicate liver disease, bone disorders, or other health conditions .
- Biochemical Research: Used to study enzyme kinetics, substrate specificity, and regulatory mechanisms.
- Therapeutic Use: Investigated for potential therapeutic applications in conditions such as hypophosphatasia, a rare genetic disorder affecting bone mineralization .
Regulatory Mechanisms
The activity of alkaline phosphatase is regulated by various factors, including:
- Phosphate Levels: High phosphate levels can inhibit the enzyme’s activity.
- Hormones: Hormones such as parathyroid hormone (PTH) and vitamin D can influence the expression and activity of alkaline phosphatase.
- Post-Translational Modifications: Glycosylation and other modifications can affect the enzyme’s stability and function .
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