ALPL Human

Alkaline Phosphatase Human Recombinant
Cat. No.
BT27023
Source

HEK293.

Synonyms

Alkaline phosphatase liver/bone/kidney isozyme, phosphoamidase, Phosphocreatine phosphatase, aalkaline phosphatase, tissue-nonspecific isozyme isoform 1, ALPL, AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ALPL Human Recombinant produced in HEK293 is a single, glycosylated polypeptide chain containing 493 amino acids (18-501 a.a) and having a molecular mass of 54.3kDa. ALPL is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Description
ALPL Human Recombinant, produced in HEK293, is a single, glycosylated polypeptide chain containing 493 amino acids (amino acids 18-501). It has a molecular mass of 54.3 kDa. ALPL is fused to a 6 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
ALPL protein solution at a concentration of 1 mg/ml in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store the vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE.
Biological Activity
The specific activity is greater than 40,000 pmol/min/µg. Specific activity is defined as the amount of enzyme required to hydrolyze 1 picomole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at a pH of 8.8 and a temperature of 37°C.
Synonyms

Alkaline phosphatase liver/bone/kidney isozyme, phosphoamidase, Phosphocreatine phosphatase, aalkaline phosphatase, tissue-nonspecific isozyme isoform 1, ALPL, AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP.

Source

HEK293.

Amino Acid Sequence

DGSLVPEKEK DPKYWRDQAQ ETLKYALELQ KLNTNVAKNV IMFLGDGMGV STVTAARILK GQLHHNPGEE TRLEMDKFPF VALSKTYNTN AQVPDSAGTA TAYLCGVKAN EGTVGVSAAT ERSRCNTTQG NEVTSILRWA KDAGKSVGIV TTTRVNHATP SAAYAHSADR DWYSDNEMPP EALSQGCKDI AYQLMHNIRD IDVIMGGGRK YMYPKNKTDV EYESDEKARG TRLDGLDLVD TWKSFKPRYK HSHFIWNRTE LLTLDPHNVD YLLGLFEPGD MQYELNRNNV TDPSLSEMVV VAIQILRKNP KGFFLLVEGG RIDHGHHEGK AKQALHEAVE MDRAIGQAGS LTSSEDTLTV VTADHSHVFT FGGYTPRGNS IFGLAPMLSD TDKKPFTAIL YGNGPGYKVV GGERENVSMV DYAHNNYQAQ SAVPLRHETH GGEDVAVFSK GPMAHLLHGV HEQNYVPHVM AYAACIGANL GHCAPAS HHHHHH.

Product Science Overview

Types and Isozymes

In humans, there are four distinct genes encoding alkaline phosphatases:

  1. ALPL: Encodes the tissue-nonspecific isozyme, also known as liver/bone/kidney alkaline phosphatase (TNAP).
  2. ALPI: Encodes the intestinal isozyme.
  3. ALPP: Encodes the placental isozyme.
  4. ALPPL2: Encodes the placental-like or germ cell isozyme .
Recombinant Human Alkaline Phosphatase

Recombinant human alkaline phosphatase (rhALP) is produced using recombinant DNA technology, which involves inserting the gene encoding ALP into a host cell, such as HEK293 cells, to produce the enzyme in large quantities. This recombinant enzyme is often tagged with a polyhistidine tag to facilitate purification .

Applications

Recombinant human alkaline phosphatase has several applications:

  • Research: Used in studies involving dephosphorylation processes and enzyme kinetics.
  • Clinical: Investigated for therapeutic potential in conditions like sepsis-associated acute kidney injury (SA-AKI). For instance, ilofotase alfa, a recombinant human alkaline phosphatase, has shown reno-protective effects in clinical trials .
  • Diagnostics: Employed in assays to measure ALP activity in blood, which can indicate liver disease or bone disorders .
Stability and Storage

Recombinant human alkaline phosphatase is typically provided as a lyophilized powder and is stable for up to twelve months when stored at -20°C to -80°C. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles .

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