ALPP Human, Active
3 ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALP, PLAP, Alkaline phosphatase placental type, EC 3.1.3.1, PLAP-1, Alkaline phosphatase Regan isozyme.
Greater than 95.0% as determined by SDS-PAGE.
Description
ALPP Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 494 amino acids (23-506 a.a.) and having a molecular mass of 53.9kDa. ALPP is expressed with a 10 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Placental alkaline phosphatase (PLAP) is a membrane-bound enzyme primarily found in the placenta during the third trimester of pregnancy. While initially thought to be exclusive to the placenta, a variant with significant similarity to PLAP has been identified. PLAP is predominantly expressed in the placenta, cervix, fallopian tubes, and specific tumors, including those in the ovaries, gastrointestinal tract, germ cells, and seminomas.
Human ALPP, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular weight of 53.9 kDa. It consists of 494 amino acids (23-506 a.a.) and includes a 10 amino acid His tag at the C-terminus. Purification is achieved through proprietary chromatographic methods.
The ALPP protein solution is provided at a concentration of 0.5 mg/ml and is prepared in a Phosphate-Buffered Saline (pH 7.4) solution containing 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be kept at 4°C. For longer storage, freezing at -20°C is recommended. Adding a carrier protein like HSA or BSA (0.1%) is advised for extended storage. To maintain product integrity, avoid repeated freezing and thawing.
SDS-PAGE analysis confirms a purity exceeding 95%.
The specific activity, exceeding 2,500 units/mg, is determined by measuring the enzyme's ability to hydrolyze p-nitrophenyl phosphate (pNPP) at a rate of 1.0 nmole per minute at pH 7.5 and a temperature of 37°C.
3 ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALP, PLAP, Alkaline phosphatase placental type, EC 3.1.3.1, PLAP-1, Alkaline phosphatase Regan isozyme.
ADLMIIPVEE ENPDFWNREA AEALGAAKKL QPAQTAAKNL IIFLGDGMGV STVTAARILK GQKKDKLGPE LPLAMDRFPY VALSKTYNVD KHVPDSGATA TAYLCGVKGN FQTIGLSAAA RFNQCNTTRG NEVISVMNRA KKAGKSVGVV TTTRVQHASP AGTYAHTVNR NWYSDADVPA SARQEGCQDI ATQLISNMDI DVILGGGRKY MFRMGTPDPE YPDDYSQGGT RLDGKNLVQE WLAKRQGARY VWNRTELMQA SLDPSVTHLM GLFEPGDMKY EIHRDSTLDP SLMEMTEAAL RLLSRNPRGF FLFVEGGRID HGHHESRAYR ALTETIMFDD AIERAGQLTS EEDTLSLVTA DHSHVFSFGG YPLRGSSIFG LAPGKARDRK AYTVLLYGNG PGYVLKDGAR PDVTESESGS PEYRQQSAVP LDEETHAGED VAVFARGPQA HLVHGVQEQT FIAHVMAFAA CLEPYTACDL APPAGTTDHH HHHH.
Product Science Overview
ALPP is a single, glycosylated polypeptide chain consisting of 494 amino acids, with a molecular mass of approximately 53.9 kDa . The enzyme is expressed with a 10 amino acid His tag at the C-terminus, which aids in its purification through chromatographic techniques . The recombinant form of ALPP is produced in Sf9 Baculovirus cells, ensuring high purity and activity .
The specific activity of ALPP is greater than 2,500 units per milligram, defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 and 37°C . This high level of activity makes it a valuable tool in various biochemical assays and research applications.
ALPP is provided as a sterile, filtered, colorless solution formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol . For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the enzyme at -20°C with the addition of a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent multiple freeze-thaw cycles .
ALPP is widely used in research due to its role in dephosphorylation processes. It is particularly useful in studies involving placental biology, cancer research, and enzyme kinetics. The enzyme is also employed in diagnostic assays to detect placental and germ cell tumors, as its expression is elevated in these conditions .
