a-Actinin
Actinin Alpha
Cat. No.
BT418
Source
Chicken Gizzard.
Synonyms
Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, Non-muscle alpha-actinin-1, F-actin cross-linking protein, ACTN1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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Description
Ultra pure Alpha Actinin having a Molecular mass of 95,000 Dalton.
Product Specs
Introduction
ACTN1, which codes for a nonmuscle, cytoskeletal, alpha actinin isoform, shares a map location with the structurally similar erythroid beta spectrin gene. Alpha actinins are part of the spectrin gene superfamily, a diverse group of cytoskeletal proteins that includes alpha and beta spectrins and dystrophins. Alpha actinin, an actin-binding protein, plays multiple roles across different cell types. The cytoskeletal isoform in nonmuscle cells is found along microfilament bundles and adherens-type junctions, where it facilitates the binding of actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, contributing to the anchoring of myofibrillar actin filaments.
Description
Highly purified Alpha Actinin with a molecular weight of 95 kDa.
Physical Appearance
Sterile-filtered, white, lyophilized powder.
Formulation
The protein was lyophilized from a solution of 1 mg/ml in 10 mM Tris acetate buffer (pH 7.6), 0.1 mM EDTA, 2 mM DTT, and 20 mM NaCl.
Solubility
For reconstitution of the lyophilized a-Actinin, it is recommended to use sterile 18 MΩ-cm H2O at a concentration of at least 100 μg/ml. Further dilutions can be made in other aqueous solutions.
Stability
Lyophilized a-Actinin remains stable at room temperature for up to 3 weeks. However, it is recommended to store it desiccated at a temperature below -18 °C. After reconstitution, a-Actinin should be stored at 4 °C for 2-7 days. For long-term storage, freezing at -18 °C is advised. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze-thaw cycles.
Applications
Used as a protein standard in 1D and 2D SDS gel electrophoresis, immunoassays, and immunization.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, Non-muscle alpha-actinin-1, F-actin cross-linking protein, ACTN1.
Source
Chicken Gizzard.
Product Science Overview
Structure
Actinin Alpha is an actin-binding protein that forms an anti-parallel rod-shaped dimer. Each end of the rod contains an actin-binding domain, which allows it to cross-link actin filaments . The structure of Actinin Alpha includes:
- N-terminal Actin Binding Domain (ABD): This domain is composed of two calponin homology (CH) domains, which are crucial for binding to actin filaments .
- Rod Domain: This domain consists of multiple spectrin repeats, providing the necessary length and flexibility for the protein .
- C-terminal Calmodulin-like (CaM) Domain: This domain is involved in the regulation of the protein’s activity through interactions with calcium ions .
Types and Isoforms
There are four genes encoding Actinin Alpha in mammals, producing at least six different protein isoforms. These isoforms can be categorized into two groups:
Functions
Actinin Alpha serves several critical functions in cells:
- Cross-linking Actin Filaments: It stabilizes the contractile apparatus in muscle cells by cross-linking actin filaments .
- Cell Signaling: It acts as a scaffold connecting different proteins in the cytoskeleton, playing a role in various cell signaling pathways .
- Cell Adhesion: It is involved in the attachment of actin filaments to the cell membrane at adhesion sites .
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