ACTN1 Human

Actinin Alpha 1 Human Recombinant
Cat. No.
BT22565
Source
Escherichia Coli.
Synonyms
ACTN1, Actinin, Alpha 1, Alpha-Actinin Cytoskeletal Isoform, F-Actin Cross-Linking Protein, Non-Muscle Alpha-Actinin-1, BDPLT15, Actinin 1 Smooth Muscle, Alpha-Actinin-1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ACTN1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 274 amino acids (1-249 a.a) and having a molecular mass of 31.4kDa. ACTN1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The ACTN1 gene provides instructions for making a non-muscle cytoskeletal protein called alpha-actinin-1. This protein is part of a family of proteins that are important for maintaining the structure and function of cells. Alpha-actinin-1 is found in various cell types and plays a crucial role in connecting actin filaments, which are essential components of the cytoskeleton, to the cell membrane. It is also involved in cell adhesion and motility. Mutations in the ACTN1 gene have been linked to various conditions, including congenital myopathy and cardiomyopathy.
Description
ACTN1 Human Recombinant protein is produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 274 amino acids (specifically, amino acids 1 to 249). This protein has a molecular mass of 31.4 kDa. For purification and detection purposes, a 25 amino acid His-tag is fused to the N-terminus of the protein. The purification process involves proprietary chromatographic techniques.
Physical Appearance
The product is a clear and colorless solution that has been sterilized by filtration.
Formulation
The ACTN1 protein is supplied in a solution with a concentration of 1 mg/ml. This solution is prepared with Phosphate Buffered Saline (PBS) at a pH of 7.4 and contains 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept refrigerated at 4°C. For longer-term storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (at a concentration of 0.1%). It's important to avoid subjecting the product to repeated cycles of freezing and thawing.
Purity
Analysis by SDS-PAGE indicates that the purity of this product is greater than 95%.
Synonyms
ACTN1, Actinin, Alpha 1, Alpha-Actinin Cytoskeletal Isoform, F-Actin Cross-Linking Protein, Non-Muscle Alpha-Actinin-1, BDPLT15, Actinin 1 Smooth Muscle, Alpha-Actinin-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMDHYD SQQTNDYMQP EEDWDRDLLL DPAWEKQQRK TFTAWCNSHL RKAGTQIENI EEDFRDGLKL MLLLEVISGE RLAKPERGKM RVHKISNVNK ALDFIASKGV KLVSIGAEEI VDGNVKMTLG MIWTIILRFA IQDISVEETS AKEGLLLWCQ RKTAPYKNVN IQNFHISWKD GLGFCALIHR HRPELIDYGK LRKDDPLTNL NTAFDVAEKY LDIPKMLDAE DIVGTARPDE KAIMTYVSSF YHAF.

Product Science Overview

Structure and Function

Alpha-actinin-1 is an F-actin cross-linking protein that plays a crucial role in the organization of the cytoskeleton . It is involved in anchoring actin filaments to various intracellular structures, thereby contributing to the stability and integrity of the cytoskeleton . The protein is known for its ability to form dimers, which are essential for its function in cross-linking actin filaments .

Gene Location and Expression

The ACTN1 gene is located on chromosome 14 in humans . It is expressed in various tissues, including the saphenous vein, ascending aorta, right coronary artery, popliteal artery, tibial arteries, and stromal cells of the endometrium . The expression pattern of ACTN1 suggests its significant role in vascular and smooth muscle tissues .

Recombinant Production

Recombinant Human Alpha-actinin-1 is produced using Escherichia coli expression systems . The recombinant protein typically includes a His tag at the N-terminus for purification purposes and is available in high purity (>95%) suitable for applications such as SDS-PAGE and mass spectrometry . This recombinant form is used in various research applications to study the protein’s function and interactions.

Biological Significance

Alpha-actinin-1 is involved in several biological processes, including:

  • Actin filament organization: It helps in the formation and maintenance of actin filament networks .
  • Focal adhesion assembly: It plays a role in the formation of focal adhesions, which are critical for cell adhesion and signaling .
  • Platelet function: It is involved in platelet degranulation, formation, and aggregation, highlighting its importance in hemostasis .
Clinical Relevance

Mutations or dysregulation of the ACTN1 gene can lead to various disorders, including platelet-related abnormalities and cytoskeletal defects . Understanding the function and regulation of Alpha-actinin-1 is crucial for developing therapeutic strategies for these conditions.

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