Actin Rabbit
Description
The purification method used (according to Spudich & Watts) results in a highly purified protein having a Molecular mass of 43,000 dalton.
Product Specs
To reconstitute the lyophilized actin, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 1 mg/ml. The resulting solution can then be further diluted in other aqueous solutions as needed.
Store the lyophilized actin at a temperature between 2-8 °C. Avoid freezing. After reconstitution, the actin solution should be stored at 4 °C and used within 2-7 days. For long-term storage, freeze the reconstituted actin solution below -18 °C.
Repeated freezing and thawing should be avoided.
Product Science Overview
In mammals, there are six known actin isoforms, which can be broadly categorized into muscle and non-muscle actins. The non-muscle actins, β-actin and γ-actin, are ubiquitously expressed in various cell types and are essential for maintaining the cytoskeleton’s integrity and facilitating cell movement . The muscle actins, including α-cardiac, α-skeletal, and smooth muscle actins, are primarily found in muscle tissues and are involved in muscle contraction.
β-Actin is a major component of the cytoskeleton and is involved in various cellular processes, including cell division, signaling, and motility. It is encoded by the ACTB gene and is highly conserved across different species . Mutations in the ACTB gene can lead to various disorders, including Baraitser-Winter syndrome, which is characterized by intellectual disability and distinctive facial features .
Rabbit monoclonal antibodies against β-actin are widely used in research to study actin’s role in cellular processes. These antibodies are produced by immunizing rabbits with a synthetic peptide corresponding to residues near the amino-terminus of human β-actin protein . The resulting antibodies are highly specific and do not cross-react with other actin isoforms, making them valuable tools for studying β-actin in various experimental settings .
Rabbit monoclonal antibodies against β-actin are used in various applications, including Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry . These antibodies are essential for detecting and quantifying β-actin in different cell types and tissues, providing insights into actin’s role in cellular processes and disease mechanisms.
