ACTA1 Antibody

In mammals, there are six known isoforms of actin:

1. α-skeletal actin: Found in skeletal muscles.
2. α-cardiac actin: Present in cardiac muscles.
3. α-smooth muscle actin: Located in vascular smooth muscles.
4. γ-smooth muscle actin: Found in enteric smooth muscles.
5. β-actin: Ubiquitously expressed in non-muscle cells.
6. γ-actin: Also expressed in non-muscle cells.

The β- and γ-actin isoforms, known as cytoplasmic actins, are highly homologous and differ by only four amino acids. These isoforms are involved in maintaining cell structure and motility .

Mouse anti-human actin antibodies are monoclonal antibodies produced by immunizing mice with synthetic peptides corresponding to human actin sequences. These antibodies are widely used in research to detect and study actin in various applications, including Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry.

One commonly used mouse anti-human actin antibody is the β-Actin (8H10D10) Mouse mAb. This antibody detects endogenous levels of total β-actin protein and may cross-react with cytoplasmic γ-actin due to the high sequence identity between these isoforms .

Mouse anti-human actin antibodies are valuable tools in cell biology research. They are used to:

• Western Blotting: Detect actin in cell lysates and tissue extracts.
• Immunohistochemistry: Visualize actin distribution in tissue sections.
• Immunofluorescence: Study actin organization in cultured cells.
• Flow Cytometry: Analyze actin expression in fixed and permeabilized cells.

These antibodies are specific to human, mouse, and rat actin and do not cross-react with other actin isoforms such as α-skeletal, α-cardiac, or α-smooth muscle actin .

Mouse anti-human actin antibodies are typically supplied in a buffer containing sodium HEPES, NaCl, BSA, glycerol, and sodium azide. They should be stored at -20°C and should not be aliquoted to maintain stability .