ACTB Antibody
Actin Beta, PS1TP5-Binding Protein 1, Beta Cytoskeletal Actin, Actin, Cytoplasmic 1, Beta-Actin, PS1TP5BP1, BRWS1, ACTB.
Description
Product Specs
Belonging to a family of six different isoforms, Actin Beta (ACTB) is one of two nonmuscle cytoskeletal actins found in humans. These actins are highly conserved proteins essential for cell motility, structure, and integrity. Alpha actins are crucial for the contractile apparatus.
The product is a clear and colorless solution that has been sterilized through filtration.
This solution has a concentration of 1mg/ml and contains the following components: PBS (pH 7.4), 10% Glycerol, and 0.02% Sodium Azide.
For short-term storage (up to 1 month), keep the product refrigerated at 4°C. For long-term storage, it is recommended to store at -20°C. Avoid repeated freeze-thaw cycles.
The product remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
This ACTB antibody has undergone rigorous testing using ELISA, Western blot analysis, and ICC/IF techniques to ensure its specificity and reactivity. However, optimal results may require titration depending on the specific application. Therefore, users are encouraged to optimize the reagent concentration for their particular experimental conditions.
Actin Beta, PS1TP5-Binding Protein 1, Beta Cytoskeletal Actin, Actin, Cytoplasmic 1, Beta-Actin, PS1TP5BP1, BRWS1, ACTB.
ACTB antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT4G6AT.
Anti-human ACTB mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ACTB amino acids 1-375 purified from E. coli.
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Actin Beta (β-Actin) is a highly conserved protein that plays a crucial role in cell motility, structure, and integrity. It is ubiquitously expressed in all eukaryotic cells and is a major component of the cytoskeleton. The use of antibodies targeting β-Actin, such as Mouse Anti-Human β-Actin antibodies, is common in various scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA, and Flow Cytometry .
β-Actin is one of the six isoforms of actin found in mammals. It is a cytoplasmic actin isoform, along with γ-actin, and these two isoforms are involved in controlling cell structure and motility . The protein sequence of β-actin is highly homologous to that of γ-actin, differing by only four biochemically similar amino acids . This high sequence identity means that antibodies raised against β-actin may cross-react with γ-actin.
Actin exists mainly as a fibrous polymer (F-actin) and can be depolymerized into its monomeric form (G-actin) in response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress .
Mouse Anti-Human β-Actin antibodies are widely used as loading controls in Western Blot experiments due to the consistent expression of β-actin in most cell types . These antibodies are also used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC) to visualize the distribution of β-actin within cells and tissues .
Mouse Anti-Human β-Actin antibodies are designed to detect endogenous levels of total β-actin protein. Due to the high sequence identity between β-actin and γ-actin, these antibodies may cross-react with cytoplasmic γ-actin . However, they do not cross-react with other actin isoforms such as α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms .
