ALDH5A1 Human
Aldehyde Dehydrogenase 5 A1 Human Recombinant
Cat. No.
BT7538
Source
Escherichia Coli.
Synonyms
Succinate-semialdehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic semialdehyde dehydrogenase, ALDH5A1, SSADH, SSDH.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ALDH5A1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 509 amino acids (48-535 a.a.) and having a molecular mass of 54.6kDa. The ALDH5A1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
ALDH5A1, a mitochondrial enzyme, plays a crucial role in the detoxification of acetaldehyde, a byproduct of alcohol metabolism. This enzyme, belonging to the aldehyde dehydrogenase family, catalyzes the NAD(+)-dependent oxidation of succinic semialdehyde. ALDH5A1 is involved in various metabolic processes, including lipid peroxidation and the metabolism of corticosteroids, biogenic amines, and neurotransmitters. Its deficiency leads to 4-hydroxybutyricaciduria, a rare genetic disorder characterized by the accumulation of 4-hydroxybutyric acid (GHB) in bodily fluids.
Description
This product consists of the human ALDH5A1 protein, recombinantly produced in E. coli. It is a single, non-glycosylated polypeptide chain, with a His tag attached to its N-terminus. The protein encompasses amino acids 48 to 535, resulting in a molecular weight of 54.6 kDa. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ALDH5A1 protein is supplied at a concentration of 0.5 mg/ml in a buffer solution containing 20mM Tris-HCl (pH 8.0), 10% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.
Stability
For short-term storage (up to four weeks), the solution can be kept at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for extended storage. It is crucial to avoid repeated freezing and thawing of the solution.
Purity
The purity of the ALDH5A1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Succinate-semialdehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic semialdehyde dehydrogenase, ALDH5A1, SSADH, SSDH.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAGRLAGLSA ALLRTDSFVG GRWLPAAATF PVQDPASGAA LGMVADCGVR EARAAVRAAY EAFCRWREVS AKERSSLLRK WYNLMIQNKD DLARIITAES GKPLKEAHGE ILYSAFFLEW FSEEARRVYG DIIHTPAKDR RALVLKQPIG VAAVITPWNF PSAMITRKVG AALAAGCTVV VKPAEDTPFS ALALAELASQ AGIPSGVYNV IPCSRKNAKE VGEAICTDPL VSKISFTGST TTGKILLHHA ANSVKRVSME LGGLAPFIVF DSANVDQAVA GAMASKFRNT GQTCVCSNQF LVQRGIHDAF VKAFAEAMKK NLRVGNGFEE GTTQGPLINE KAVEKVEKQV NDAVSKGATV VTGGKRHQLG KNFFEPTLLC NVTQDMLCTH EETFGPLAPV IKFDTEEEAI AIANAADVGL AGYFYSQDPA QIWRVAEQLE VGMVGVNEGL ISSVECPFGG VKQSGLGREG SKYGIDEYLE LKYVCYGGL.
Product Science Overview
Function and Importance
ALDH5A1 mediates the NADP±dependent oxidation of aldehydes into acids . This function is vital for several metabolic processes, including:
- Detoxification of Alcohol-Derived Acetaldehyde: ALDH5A1 helps in converting acetaldehyde, a toxic byproduct of alcohol metabolism, into less harmful substances .
- Lipid Peroxidation: It plays a role in the breakdown of lipid peroxides, which are harmful byproducts of lipid metabolism .
- Metabolism of Corticosteroids, Biogenic Amines, and Neurotransmitters: ALDH5A1 is involved in the metabolism of various important biological molecules, contributing to the regulation of physiological processes .
Expression and Structure
ALDH5A1 is expressed in a variety of tissues, including the liver, heart, lung, brain, kidney, and placenta . The recombinant form of this protein is typically produced in Escherichia coli (E. coli) and purified using conventional chromatography techniques .
The recombinant human ALDH5A1 protein is often fused with a His-tag at the N-terminus, which facilitates its purification and detection . The protein consists of 509 amino acids, corresponding to the amino acids 48-535 of the native human ALDH5A1 . It has a molecular mass of approximately 54.6 kDa .
Applications
Recombinant ALDH5A1 is primarily used for research purposes. It is utilized in studies related to:
- Enzyme Function and Mechanism: Understanding how ALDH5A1 functions at a molecular level.
- Drug Development: Investigating potential inhibitors or activators of ALDH5A1 for therapeutic purposes.
- Metabolic Pathways: Exploring the role of ALDH5A1 in various metabolic pathways and its impact on human health.
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