ALDH3A1 Human
Aldehyde Dehydrogenase 3 Family Member A1 Human Recombinant
Cat. No.
BT7475
Source
Escherichia Coli.
Synonyms
Aldehyde dehydrogenase 3 family member A1, aldehyde dehydrogenase, dimeric NADP-preferring, ALDH-3, aldehyde dehydrogenase isozyme, ALDHIII, MGC104062, aldehyde dehydrogenase type III, Aldehyde dehydrogenase,
stomach aldehyde dehydrogenase, EC 1.2.1.53, aldehyde dehydrogenase 3A, Aldehyde dehydrogenase.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ALDH3A1 Human Recombinant produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 473 amino acids (1-453 a.a.) and having a molecular mass of 52.5 kDa. ALDH3A1 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
ALDH3A1, an enzyme crucial for detoxifying alcohol-derived acetaldehyde, plays a vital role in metabolizing various substances such as corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation products. It exhibits activity against aromatic aldehyde substrates and toxic aldehydes. Functioning as a cytoplasmic homodimer, ALDH3A1 oxidizes aromatic, medium-chain saturated, and unsaturated aldehydes. Moreover, it contributes to corneal protection by enhancing resistance against UV and 4-hydroxy-2-nonenal-induced oxidative stress.
Description
Recombinantly produced in E. coli, ALDH3A1 Human Recombinant is a single, non-glycosylated polypeptide chain comprising 473 amino acids (specifically, amino acids 1 to 453). With a molecular weight of 52.5 kDa, this protein features a 20 amino acid His Tag fused at its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ALDH3A1 solution is prepared with 20mM Tris-HCl at a pH of 8, 0.1M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing cycles should be avoided.
Purity
SDS-PAGE analysis confirms a purity exceeding 95%.
Biological Activity
The specific activity is determined to be less than 1 unit/ml. This activity is measured by monitoring the increase in NADP absorbance at 340 nm, resulting from NAD reduction. One unit signifies the oxidation of 1 µmole of acetaldehyde to acetic acid per minute at a pH of 8 and a temperature of 25°C, in the presence of beta-NAD, potassium, and thiols.
Synonyms
Aldehyde dehydrogenase 3 family member A1, aldehyde dehydrogenase, dimeric NADP-preferring, ALDH-3, aldehyde dehydrogenase isozyme, ALDHIII, MGC104062, aldehyde dehydrogenase type III, Aldehyde dehydrogenase,
stomach aldehyde dehydrogenase, EC 1.2.1.53, aldehyde dehydrogenase 3A, Aldehyde dehydrogenase.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNAVVLKPS ELSENMASLL ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVLPIVCVRSLEE AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM TQH.
Product Science Overview
Gene and Protein Structure
Function and Activity
- Detoxification: ALDH3A1 is involved in the detoxification of alcohol-derived acetaldehyde, a toxic byproduct of alcohol metabolism . It also participates in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation .
- Oxidation: The enzyme oxidizes various aldehydes to their corresponding acids, reducing their toxicity .
- Protection: It is thought to promote resistance to UV and oxidative damage, particularly in the cornea .
Expression and Localization
- Tissue Expression: ALDH3A1 is highly expressed in the cornea of mammalian species, where it can constitute up to 50% of the soluble protein content . It is also expressed in other tissues such as the nasal epithelium, trachea, and oral cavity .
- Cellular Localization: The enzyme is found in the cytoplasm and is an integral component of the plasma membrane and endoplasmic reticulum .
Clinical Significance
- Disease Association: Mutations or dysregulation of ALDH3A1 have been associated with conditions such as keratoconus and Sabinas brittle hair syndrome .
- Therapeutic Potential: Due to its role in detoxification and protection against oxidative damage, ALDH3A1 is a potential target for therapeutic interventions in diseases related to oxidative stress .
Recombinant ALDH3A1
- Production: Human recombinant ALDH3A1 is produced using recombinant DNA technology, typically expressed in E. coli . This allows for the production of large quantities of the enzyme for research and therapeutic purposes.
- Applications: Recombinant ALDH3A1 is used in various biochemical assays to study its function and potential therapeutic applications .
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