ALDH5A1 Antibody

Aldehyde Dehydrogenase 5 A1, Mouse Anti Human
Cat. No.
BT13693
Source
Synonyms
Succinate-semialdehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic semialdehyde dehydrogenase, ALDH5A1, SSADH, SSDH.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
ALDH5A1, a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase belonging to the aldehyde dehydrogenase protein family, plays a crucial role in detoxifying alcohol-derived acetaldehyde. This enzyme mediates the NADP+-dependent oxidation of aldehydes into acids, contributing to lipid peroxidation and the metabolism of corticosteroids, biogenic amines, and neurotransmitters. Expressed in various tissues like the liver, heart, lung, brain, kidney, and placenta, ALDH5A1 deficiency leads to 4-hydroxybutyricaciduria, a rare genetic disorder. This deficiency disrupts the metabolism of the neurotransmitter 4-aminobutyric acid (GABA), causing an accumulation of GHB, a compound with significant neuromodulatory effects, in bodily fluids.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The solution contains 1mg/ml of ALDH5A1 antibody in a buffer consisting of PBS (pH 7.4), 10% glycerol, and 0.02% sodium azide.
Storage Procedures
Store at 4°C for up to 1 month. For extended storage, keep at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, Flow cytometry, and ICC/IF, to confirm its specificity and reactivity. However, it is recommended to perform individual titrations for each experiment to determine the optimal antibody concentration for specific applications.
Synonyms
Succinate-semialdehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic semialdehyde dehydrogenase, ALDH5A1, SSADH, SSDH.
Purification Method
ALDH5A1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT17F5AT.
Immunogen
Anti-human ALDH5A1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ALDH5A1 protein 48-535 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.

Product Science Overview

Introduction

Aldehyde Dehydrogenase 5 A1 (ALDH5A1) is a crucial enzyme involved in the metabolism of gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter in the central nervous system. This enzyme plays a significant role in maintaining the balance of neurotransmitters and ensuring proper neurological function.

ALDH5A1 Function and Importance

ALDH5A1 is responsible for the oxidation of succinic semialdehyde to succinate, a key step in the GABA degradation pathway. This process is essential for preventing the accumulation of toxic levels of GABA and its metabolites, which can lead to neurological disorders. Deficiency in ALDH5A1 activity results in a rare autosomal recessive disorder known as Succinic Semialdehyde Dehydrogenase (SSADH) deficiency . This condition is characterized by developmental delays, hypotonia, and seizures due to the accumulation of GABA and gamma-hydroxybutyric acid (GHB).

Mouse Anti-Human ALDH5A1 Antibody

Mouse anti-human ALDH5A1 antibodies are monoclonal antibodies specifically designed to target and bind to the human ALDH5A1 protein. These antibodies are widely used in research and diagnostic applications to study the expression and function of ALDH5A1 in various biological samples.

Applications
  1. Western Blotting (WB): Used to detect ALDH5A1 protein levels in tissue and cell lysates.
  2. Immunoprecipitation (IP): Helps in isolating ALDH5A1 protein complexes from biological samples.
  3. Immunofluorescence (IF): Allows visualization of ALDH5A1 localization within cells.
  4. Immunohistochemistry (IHC): Used to study ALDH5A1 expression in tissue sections.
  5. Enzyme-Linked Immunosorbent Assay (ELISA): Quantifies ALDH5A1 protein levels in various samples.
Research and Clinical Significance

The study of ALDH5A1 and its associated antibodies has significant implications in understanding neurological disorders and developing potential therapeutic interventions. Research on ALDH5A1 can provide insights into the mechanisms underlying SSADH deficiency and other related conditions. Additionally, these antibodies can be used to develop diagnostic tools for early detection and monitoring of neurological diseases.

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