AKT1 Human, Sf9
Protein Kinase B Alpha Human Recombinant, Sf9
Cat. No.
BT8648
Source
Sf9,
Baculovirus cells.
Synonyms
V-Akt Murine Thymoma Viral Oncogene Homolog 1, Protein
Kinase B Alpha, Proto-Oncogene C-Akt, RAC-PK-Alpha, EC 2.7.11.1, PKB Alpha,
CWS6, PKB, RAC, RAC-Alpha Serine/Threonine-Protein Kinase, Rac Protein Kinase
Alpha, Protein Kinase B, PKB-ALPHA, RAC-ALPHA, EC 2.7.11, AKT1m, PRKBA, AKT,
RAC-alpha serine/threonine-protein kinase.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
AKT1 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 488 amino acids (1-480a.a.) and
having a molecular mass of 56.7kDa. (Molecular size on SDS-PAGE will appear at
approximately 50-70kDa).
AKT1 is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Akt1, also called Akt or protein kinase B (PKB), is a crucial molecule for cell signaling in mammals. Humans possess three genes within the "Akt family": Akt1, Akt2, and Akt3. These enzymes belong to the serine/threonine-specific protein kinase family (EC2.7.11.1). Akt1 plays a vital role in cell survival by suppressing apoptotic processes. Additionally, it can stimulate protein synthesis pathways, making it a critical signaling protein for muscle hypertrophy and tissue growth. Its ability to inhibit apoptosis and promote cell survival has implicated Akt1 as a significant factor in various cancers. Initially, Akt (now referred to as Akt1) was identified as the oncogene in the AKT8 transforming retrovirus.
Description
Produced in Sf9 Baculovirus cells, AKT1 is a single, glycosylated polypeptide chain consisting of 488 amino acids (1-480a.a.) with a molecular mass of 56.7kDa. On SDS-PAGE, its molecular size appears approximately between 50-70kDa. The AKT1 protein is expressed with an 8 amino acid His tag at its C-Terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The AKT1 protein solution is provided at a concentration of 0.5mg/ml and contains phosphate buffered saline (pH 7.4) and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the AKT1 protein should be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. To maintain protein stability, avoid repeated freeze-thaw cycles.
Purity
The purity of the AKT1 protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
V-Akt Murine Thymoma Viral Oncogene Homolog 1, Protein
Kinase B Alpha, Proto-Oncogene C-Akt, RAC-PK-Alpha, EC 2.7.11.1, PKB Alpha,
CWS6, PKB, RAC, RAC-Alpha Serine/Threonine-Protein Kinase, Rac Protein Kinase
Alpha, Protein Kinase B, PKB-ALPHA, RAC-ALPHA, EC 2.7.11, AKT1m, PRKBA, AKT,
RAC-alpha serine/threonine-protein kinase.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
MSDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA
PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KKQEEEEMDF RSGSPSDNSG
AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI
LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA
NGGELFFHLS RERVFSEDRA RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI
KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL
ILMEEIRFPR TLGPEAKSLL SGLLKKDPKQ RLGGGSEDAK EIMQHRFFAG
IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA LEHHHHHH.
Product Science Overview
Structure and Function
PKBα is composed of three main domains:
- Pleckstrin Homology (PH) Domain: This domain is responsible for binding phosphoinositides and is crucial for the membrane localization of the kinase.
- Kinase Domain: This is the catalytic domain responsible for the enzyme’s kinase activity.
- Regulatory Domain: This domain contains sites for phosphorylation which are essential for the activation of the kinase.
PKBα is activated through a series of phosphorylation events. Upon activation by upstream kinases such as PDK1 and mTORC2, PKBα translocates to the plasma membrane where it phosphorylates a variety of downstream substrates involved in cell survival, growth, and metabolism.
Recombinant Expression in Sf9 Cells
The recombinant form of PKBα is often expressed in Sf9 cells, which are derived from the fall armyworm Spodoptera frugiperda. These cells are commonly used in the baculovirus expression system, which is advantageous for producing high yields of recombinant proteins with post-translational modifications similar to those in higher eukaryotes.
Applications
Recombinant PKBα is widely used in research to study its role in various signaling pathways. It is also used in drug discovery and development, particularly in the context of cancer, as aberrant Akt signaling is implicated in many types of cancer.
Storage and Handling
Recombinant PKBα is typically stored at -20°C to maintain its stability and activity. It is important to avoid repeated freeze-thaw cycles to prevent degradation.
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