AKT1 Antibody
V-Akt Murine Thymoma Viral Oncogene Homolog 1, Protein Kinase B Alpha, Proto-Oncogene C-Akt, RAC-PK-Alpha, EC 2.7.11.1, PKB Alpha, CWS6, PKB, RAC, RAC-Alpha Serine/Threonine-Protein Kinase, Rac Protein Kinase Alpha, Protein Kinase B, PKB-ALPHA, RAC-ALPHA, EC 2.7.11, AKT1m, PRKBA, AKT, RAC-alpha serine/threonine-protein kinase.
Description
Product Specs
V-Akt Murine Thymoma Viral Oncogene Homolog 1, Protein Kinase B Alpha, Proto-Oncogene C-Akt, RAC-PK-Alpha, EC 2.7.11.1, PKB Alpha, CWS6, PKB, RAC, RAC-Alpha Serine/Threonine-Protein Kinase, Rac Protein Kinase Alpha, Protein Kinase B, PKB-ALPHA, RAC-ALPHA, EC 2.7.11, AKT1m, PRKBA, AKT, RAC-alpha serine/threonine-protein kinase.
AKT1 antibody was purified from mouse ascetic fluids by protein-A affinity chromatography.
PAT6D5AT.
Anti-human AKT1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human AKT1 amino acids 1-480 purified from E. coli.
Mouse IgG2a heavy chain and κ light chain.
Product Science Overview
Protein Kinase B was first cloned by three independent groups in 1991, following the identification of its viral homolog, the v-Akt proto-oncogene, expressed by a transforming retrovirus (AKT-8) isolated from a spontaneous thymic lymphoma of an AKR mouse . The three mammalian isoforms, Akt1, Akt2, and Akt3, share a high degree of similarity but have distinct physiological roles .
Protein Kinase B Alpha is a key mediator of the phosphoinositide 3-kinase (PI3K) signaling pathway . Upon activation by PI3K, Protein Kinase B Alpha is phosphorylated at two key residues, T308 and S473, by PDK1 and mTORC2 or DNA-PK, respectively . This phosphorylation is essential for its full kinase activity, allowing it to regulate various downstream targets involved in cell survival, growth, and metabolism .
In the context of cancer, Protein Kinase B Alpha has been shown to regulate tumor growth, survival, and invasiveness of tumor cells . It increases cell proliferation through cell cycle proteins like p21, p27, and cyclin D1 and impairs apoptosis via p53 . However, it also decreases the migration of cancer cells by regulating proteins such as TSC2, palladin, and EMT-proteins .
The Mouse Anti Human Protein Kinase B Alpha antibody is a monoclonal antibody used in research to detect and study the human form of Protein Kinase B Alpha. This antibody is produced by immunizing mice with human Protein Kinase B Alpha and then isolating the specific antibody-producing cells. The resulting monoclonal antibody can specifically bind to human Protein Kinase B Alpha, allowing researchers to investigate its expression, localization, and function in various biological samples.
The Mouse Anti Human Protein Kinase B Alpha antibody is widely used in various research applications, including:
- Western Blotting: To detect and quantify Protein Kinase B Alpha in cell and tissue lysates.
- Immunohistochemistry: To study the localization and expression patterns of Protein Kinase B Alpha in tissue sections.
- Flow Cytometry: To analyze the expression of Protein Kinase B Alpha on the surface of cells.
- Immunoprecipitation: To isolate and study Protein Kinase B Alpha and its interacting partners.
