PKAkt1/PKBa Human
Protein Kinase Akt1/PKB alpha, Inactive enzyme Human Recombinant
Cat. No.
BT8844
Source
Sf9 insect cells.
Synonyms
RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, AKT1, AKT, RAC, PRKBA, MGC99656, RAC-ALPHA.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PKAkt1 is a glycosilated polypeptide having a molecular mass of 59.1 kDa, fused with a polyhistidine tag at N-terminus (to facilitate removal of Akt1 kinase from the reaction mixture).
Inactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate.
Recombinant Protein Kinase B is purified by proprietary chromatographic techniques.
Inactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate.
Recombinant Protein Kinase B is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Akt1, alternatively called "Akt" or protein kinase B (PKB), is a crucial signaling molecule in mammals. Humans possess three genes within the "Akt family": Akt1, Akt2, and Akt3. These enzymes belong to the serine/threonine-specific protein kinase family (EC 2.7.11.1). Akt1 participates in cell survival by suppressing apoptotic processes. It also stimulates protein synthesis pathways, playing a vital role in skeletal muscle hypertrophy and overall tissue growth. Due to its ability to inhibit apoptosis and promote cell survival, Akt1 is implicated in the development of various cancers. Originally identified as the oncogene in the AKT8 transforming retrovirus, Akt was later renamed Akt1.
Description
PKAkt1, a glycosylated polypeptide with a molecular weight of 59.1 kDa, is fused with an N-terminal polyhistidine tag for efficient removal from reaction mixtures. This inactive enzyme serves as a negative control or substrate for phosphorylation studies. Recombinant Protein Kinase B is purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
PKAkt1 is supplied in a buffer containing 50mM Tris-HCl, 100mM NaCl, 1mM DTT, 25mM beta-glycerophosphate, and 50% glycerol at pH 8.5.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Minimize repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Unit Definition
One unit is defined as the amount of enzyme catalyzing the transfer of 1 picomole of phosphate to the synthetic peptide substrate (RPRAATF) per minute at 30°C.
Biological Activity
The enzyme exhibits no protease activity as assessed by the Twinning test. The specific activity is 235 U/mg.
Synonyms
RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, AKT1, AKT, RAC, PRKBA, MGC99656, RAC-ALPHA.
Source
Sf9 insect cells.
Product Science Overview
Structure and Characteristics
The recombinant form of Protein Kinase Akt1 is a glycosylated polypeptide with a molecular mass of approximately 59.1 kDa. It is fused with a polyhistidine tag at the N-terminus to facilitate the removal of Akt1 kinase from the reaction mixture . This recombinant enzyme is typically produced in Sf9 insect cells and is purified using proprietary chromatographic techniques .
Function and Mechanism
Akt1 is involved in several critical cellular pathways:
- Cell Survival and Apoptosis: Akt1 inhibits apoptotic processes, thereby promoting cell survival. It phosphorylates and inactivates components of the apoptotic machinery, such as the pro-apoptotic protein BAD .
- Metabolism: Akt1 regulates glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface . It also phosphorylates and inhibits glycogen synthase kinase 3 (GSK3), promoting glycogen synthesis .
- Protein Synthesis and Growth: Akt1 induces protein synthesis pathways, contributing to skeletal muscle hypertrophy and general tissue growth. It phosphorylates and activates mTORC1 signaling, leading to increased protein synthesis .
- Angiogenesis: Akt1 promotes angiogenesis by enhancing the expression of vascular endothelial growth factor (VEGF) and other angiogenic factors .
Inactive Enzyme and Applications
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