AGR3 Human
Anterior gradient protein 3 homolog, AG-3, AG3, hAG-3, Breast cancer membrane protein 11, AGR3, BCMP11, UNQ642/PRO1272, HAG3, PDIA18.
Description
AGR3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (22-166 a.a) and having a molecular mass of 19.5kDa.
AGR3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
The AGR3 protein, also known as Anterior Gradient Protein 3 Homolog, plays a role in the development and progression of cancer. This protein is found in the cytoplasm of cells and is secreted outside the cell. AGR3 is involved in promoting the spread of cancer cells (metastasis) and interfering with the function of the p53 protein, a crucial tumor suppressor. Due to its association with hormone-dependent breast cancers, AGR3 shows potential as both a biomarker for disease detection and a target for therapeutic interventions.
This recombinant AGR3 protein is produced in E. coli, resulting in a single polypeptide chain that lacks glycosylation. It consists of 169 amino acids, with the sequence spanning from amino acid 22 to 166. The molecular weight of the protein is 19.5 kDa. For purification purposes, a 20-amino acid His-tag is added to the N-terminus. The protein is then purified using specialized chromatographic techniques.
This AGR3 protein solution has a concentration of 1 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 20% glycerol, 0.1 M NaCl, and 1 mM DTT.
For short-term storage of 2-4 weeks, the AGR3 protein solution can be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Anterior gradient protein 3 homolog, AG-3, AG3, hAG-3, Breast cancer membrane protein 11, AGR3, BCMP11, UNQ642/PRO1272, HAG3, PDIA18.
MGSSHHHHHH SSGLVPRGSH MGSMIAIKKE KRPPQTLSRG WGDDITWVQT YEEGLFYAQK SKKPLMVIHH LEDCQYSQAL KKVFAQNEEI QEMAQNKFIM LNLMHETTDK NLSPDGQYVP RIMFVDPSLT VRADIAGRYS NRLYTYEPRD LPLLIENMKK ALRLIQSEL.
Product Science Overview
AGR3 is characterized by:
- An N-terminal ER-signal sequence.
- A catalytically active thioredoxin domain.
- A C-terminal ER-retention sequence .
This protein is predominantly expressed in ciliated airway epithelial cells and plays a crucial role in regulating ciliary beat frequency and mucociliary clearance in the airway . In mice, AGR3 is involved in the regulation of intracellular calcium in tracheal epithelial cells .
AGR3 has several important biological functions:
- Metastasis Induction: AGR3 is implicated in the induction of metastasis, making it a potential marker for cancer progression .
- p53 Tumor Suppressor Inhibition: It is known to inhibit the p53 tumor suppressor, which is a critical protein in preventing cancer development .
- Calcium-Mediated Regulation: AGR3 is required for calcium-mediated regulation of ciliary beat frequency, which is essential for maintaining mucociliary clearance in the respiratory tract .
