ADH1C Human, sf9

Alcohol Dehydrogenase 1C, Human Recombinant, sf9
Cat. No.
BT6877
Source
Sf9, Baculovirus cells.
Synonyms
ADH1, ADH1C, ADH3, Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ADH1C Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 381 amino acids (1-375) and having a molecular mass of 40.6kDa. ADH1C is fused to a 6 amino acid IgG His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
ADH1C, a member of the zinc-containing alcohol dehydrogenase family, plays a crucial role in metabolizing various substrates, including ethanol, retinol, aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This monomorphic enzyme exhibits high activity in fetal and infant livers, gradually decreasing during gestation and becoming minimally active in adulthood.
Description
ADH1C Human Recombinant, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 381 amino acids (1-375) with a molecular weight of 40.6kDa. This recombinant protein is fused to a 6 amino acid IgG His-Tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The ADH1C protein solution (1mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It is crucial to avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% based on SDS-PAGE analysis.
Synonyms
ADH1, ADH1C, ADH3, Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAAGICRSD EHVVSGNLVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLFTPQCGK CRICKNPESN YCLKNDLGNP RGTLQDGTRR FTCSGKPIHH FVGVSTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVKVAKV TPGSTCAVFG LGGVGLSVVM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSINPMLLL TGRTWKGAIF GGFKSKESVP KLVADFMAKK FSLDALITNI LPFEKINEGF DLLRSGKSIR
TVLTFHHHHH H.

Product Science Overview

Introduction

Alcohol Dehydrogenase 1C (ADH1C) is an enzyme that plays a crucial role in the metabolism of alcohols within the human body. This enzyme is part of the alcohol dehydrogenase family, which is responsible for the oxidation of alcohols to aldehydes or ketones. The recombinant form of ADH1C, produced in Sf9 cells, is a valuable tool for research and industrial applications.

Structure and Production

The recombinant ADH1C enzyme is produced in Sf9 Baculovirus cells. It is a single, glycosylated polypeptide chain containing 381 amino acids, with a molecular mass of approximately 40.6 kDa . The molecular size on SDS-PAGE appears at approximately 40-57 kDa . This recombinant form is designed to mimic the natural enzyme found in humans, allowing for detailed studies on its function and properties.

Function and Mechanism

ADH1C catalyzes the conversion of alcohols to aldehydes, a critical step in the metabolism of ethanol and other alcohols. The enzyme operates by binding to the alcohol substrate and facilitating its oxidation through the transfer of electrons. This process is essential for the detoxification of alcohols in the liver and other tissues.

Applications

The recombinant ADH1C enzyme has several applications in both research and industry:

  • Biochemical Research: It is used to study the metabolic pathways involving alcohols and aldehydes, providing insights into the detoxification processes in the human body.
  • Drug Development: ADH1C is utilized in the development of drugs targeting alcohol metabolism disorders, such as alcoholism and alcohol-induced liver disease.
  • Industrial Biotechnology: The enzyme is employed in the production of biofuels and other industrial processes involving the oxidation of alcohols.

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