ADH1A Human, sf9

Alcohol Dehydrogenase 1A, Human Recombinant, sf9
Cat. No.
BT6740
Source
Sf9, Baculovirus cells.
Synonyms
ADH1A, Alcohol dehydrogenase 1A, Alcohol dehydrogenase 1A, Alcohol dehydrogenase subunit alpha, ADH1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ADH1A Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 381 amino acids (1-375) and having a molecular mass of 40.6kDa. ADH1A is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Alcohol dehydrogenase 1A (ADH1A) is an enzyme that plays a crucial role in ethanol metabolism. It works in conjunction with the coenzyme NAD to catalyze the reversible conversion of organic alcohols to ketones or aldehydes. In the liver, ADH1A is responsible for eliminating ethanol produced by gut microorganisms. This enzyme is highly prevalent and active in fetal and infant livers, but its activity gradually declines during gestation and remains low in adulthood.
Description
Recombinant human ADH1A, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain with a molecular weight of 40.6 kDa. It comprises 381 amino acids, including a 6-amino acid His-tag fused at the C-terminus (residues 1-375). Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The ADH1A protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is buffered with phosphate-buffered saline (PBS) at a pH of 7.4 and contains 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing of the solution.
Purity
The purity of the protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
ADH1A, Alcohol dehydrogenase 1A, Alcohol dehydrogenase 1A, Alcohol dehydrogenase subunit alpha, ADH1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAVGICGTD DHVVSGTMVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLAIPQCGK CRICKNPESN YCLKNDVSNP QGTLQDGTSR FTCRRKPIHH FLGISTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVP KLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR TILMFHHHHH H.

Product Science Overview

Structure and Function

ADH1A is composed of several homo- and heterodimers of alpha, beta, and gamma subunits. The alpha subunit, encoded by the ADH1A gene, is particularly significant in the liver during early fetal development but shows only weak activity in adult liver . The enzyme’s primary function is to oxidize alcohols to aldehydes, using the coenzyme NAD (Nicotinamide Adenine Dinucleotide) as a cofactor .

Recombinant Production in sf9 Cells

The recombinant form of ADH1A is produced in Sf9 Baculovirus cells. This method involves the expression of the human ADH1A gene in insect cells, resulting in a glycosylated polypeptide chain containing 381 amino acids and having a molecular mass of approximately 40.6 kDa . The recombinant protein is often used in research to study the enzyme’s properties and its role in alcohol metabolism.

Applications and Importance

ADH1A is widely used in biochemical research to understand the mechanisms of alcohol metabolism and its effects on the human body. It is also used in studies related to substance dependence and certain personality traits, as mutations in the ADH1A gene may contribute to variations in these areas .

Physiological Role

In the liver, ADH1A helps eliminate ethanol produced by microorganisms in the intestinal tract. This detoxification process is vital for maintaining the body’s homeostasis and preventing the toxic effects of alcohol accumulation .

Genetic and Molecular Information

The ADH1A gene is located on the long arm of chromosome 4 and is part of a cluster that includes six additional alcohol dehydrogenase genes. These genes encode the different subunits of the enzyme, contributing to its diverse functionality .

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