ADH1C Human

Alcohol Dehydrogenase 1C Human Recombinant
Cat. No.
BT6791
Source
E.coli.
Synonyms
Alcohol dehydrogenase 1C (class I) gamma polypeptide, Alcohol dehydrogenase subunit gamma, alcohol dehydrogenase 3 (class I) gamma polypeptide, ADH gamma subunit, aldehyde reductase, ADH3, EC 1.1.1.1, EC 1.1.1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ADH1C Human Recombinant produced in E. coli is a single polypeptide chain containing 399 amino acids (1-375) and having a molecular mass of 42.4 kDa.
ADH1C is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ADH1C, a member of the zinc-containing alcohol dehydrogenase family, plays a crucial role in metabolizing a wide range of substrates including ethanol, retinol, aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This enzyme exhibits monomorphic behavior and is particularly vital in fetal and infant livers. However, its activity decreases during gestation and remains relatively low in adulthood.
Description
Recombinant human ADH1C, expressed in E. coli, is a single polypeptide chain comprising 399 amino acids (1-375) with a molecular weight of 42.4 kDa. This protein features a 24 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution free from particulate matter.
Formulation
The ADH1C solution is provided at a concentration of 0.5 mg/ml and is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 2 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For longer storage periods, it is recommended to store the solution frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of ADH1C is determined to be greater than 90% as assessed by SDS-PAGE analysis.
Synonyms
Alcohol dehydrogenase 1C (class I) gamma polypeptide, Alcohol dehydrogenase subunit gamma, alcohol dehydrogenase 3 (class I) gamma polypeptide, ADH gamma subunit, aldehyde reductase, ADH3, EC 1.1.1.1, EC 1.1.1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSTAGK VIKCKAAVLW ELKKPFSIEE VEVAPPKAHE VRIKMVAAGI CRSDEHVVSG NLVTPLPVIL GHEAAGIVES VGEGVTTVKP GDKVIPLFTP QCGKCRICKN PESNYCLKND LGNPRGTLQD GTRRFTCSGK PIHHFVGVST FSQYTVVDEN AVAKIDAASP LEKVCLIGCG FSTGYGSAVK VAKVTPGSTC AVFGLGGVGL SVVMGCKAAG AARIIAVDIN KDKFAKAKEL GATECINPQD YKKPIQEVLK EMTDGGVDFS FEVIGRLDTM MASLLCCHEA CGTSVIVGVP PDSQNLSINP MLLLTGRTWK GAIFGGFKSK ESVPKLVADF MAKKFSLDAL ITNILPFEKI NEGFDLLRSG KSIRTVLTF.

Product Science Overview

Structure and Function

ADH1C is a protein-coding gene that produces a single polypeptide chain containing 399 amino acids, with a molecular mass of approximately 42.4 kDa . The enzyme is characterized by its high activity for ethanol oxidation, converting ethanol to acetaldehyde, which is a key step in ethanol catabolism . This process is essential for the body’s ability to metabolize and eliminate alcohol.

The enzyme exists as several homo- and heterodimers of alpha, beta, and gamma subunits. The gamma subunit, encoded by the ADH1C gene, is one of these subunits and is known for its high activity in ethanol oxidation . The genes encoding the alpha, beta, and gamma subunits are organized in a genomic segment as a gene cluster .

Recombinant Production

Human recombinant ADH1C is produced using Escherichia coli (E. coli) expression systems. The recombinant enzyme is typically fused to a His-tag at the N-terminus to facilitate purification through chromatographic techniques . This recombinant form is used in various research and industrial applications to study the enzyme’s properties and its role in alcohol metabolism.

Clinical and Research Significance

ADH1C has been studied extensively for its role in alcohol metabolism and its association with alcohol dependence and other diseases. While an association between ADH1C polymorphism and alcohol dependence has not been firmly established, the enzyme’s activity in ethanol oxidation makes it a significant focus of research in understanding alcohol-related disorders .

Additionally, ADH1C is involved in other metabolic pathways, such as the oxidation of retinol and the metabolism of retinoic acid, which are important for various physiological processes . The enzyme’s role in these pathways highlights its broader significance beyond alcohol metabolism.

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