AZGP1 Human, Sf9

The AZGP1 gene is located on chromosome 7q22.1 in humans . The protein is widely expressed in various tissues and body fluids, including the breast, stomach, liver, prostate, plasma, urine, and saliva . The human recombinant version of this protein, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 286 amino acids (21-298 a.a) and has a molecular mass of 33.2 kDa . It is fused to an 8 amino acid His-tag at the C-terminus and purified by proprietary chromatographic techniques .

AZGP1 is known for its role in stimulating lipolysis and inducing a reduction in body fat in mice . It is associated with cachexia related to cancer and is expressed in the secretory cells of lung epithelium . Additionally, decreased circulating levels of AZGP1 are associated with type 2 diabetes .

Recent studies have investigated the immunological function of AZGP1 in regulating tumor response, particularly in the breast cancer microenvironment . AZGP1 expression has been found to be negatively correlated with multiple immunological processes and specific immune cell infiltration, including macrophages . It is suggested to be a novel immunoregulatory factor affecting the macrophage phenotype in breast cancer tissues .

AZGP1 has been identified as a biomarker in cancer patients, with its role varying depending on the specific type of cancer . For instance, smoking increases the expression of this gene, which is why smoking cessation often leads to weight gain .