YWHAG PAT4B9AT Antibody
Tyr-3/Trp-5 Monooxygenase Activation Protein Gamma Clone PAT4B9AT, Mouse Anti Human
Cat. No.
BT27900
Source
Synonyms
14-3-3 protein gamma, Protein kinase C inhibitor protein
1, KCIP-1, YWHAG, Tyrosine 3 monooxygenase/tryptophan 5-monooxygenase
activation protein gamma polypeptide.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
The 14-3-3 protein family plays a crucial regulatory function in signal transduction, checkpoint control, apoptotic, and nutrient-sensing pathways. These proteins are highly conserved and exhibit ubiquitous expression. Within mammals, there are at least seven identified isoforms. The 14-3-3 gamma isoform, a subtype within the 14-3-3 family, was initially thought to be exclusive to the brain and neurons. Studies have revealed its interaction with RAF1 and protein kinase C, both of which are proteins implicated in various signal transduction pathways.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody is supplied at a concentration of 1 mg/ml in a buffer consisting of phosphate-buffered saline (PBS) at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be kept at 4°C. For extended storage, it is recommended to store the antibody at -20°C. Repeated freezing and thawing of the antibody should be avoided.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
This antibody has undergone rigorous testing using ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions may vary depending on the specific application. Therefore, it is recommended to titrate the antibody in each experimental setup to determine the most effective concentration. A starting dilution of 1:1000 is suggested as a general guideline.
Synonyms
14-3-3 protein gamma, Protein kinase C inhibitor protein
1, KCIP-1, YWHAG, Tyrosine 3 monooxygenase/tryptophan 5-monooxygenase
activation protein gamma polypeptide.
Purification Method
YWHAG antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT4B9AT.
Immunogen
Anti-human YWHAG mAb,
clone PAT4B9AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human YWHAG protein 1-247
amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Introduction
The Tyr-3/Trp-5 Monooxygenase Activation Protein Gamma, also known as 14-3-3 protein gamma, is a member of the 14-3-3 family of proteins. These proteins are highly conserved and play crucial roles in various cellular processes, including signal transduction, cell cycle control, and apoptosis. The specific clone PAT4B9AT is a monoclonal antibody produced in mice and is used for research purposes to study human proteins.
Preparation Methods
The preparation of the Tyr-3/Trp-5 Monooxygenase Activation Protein Gamma Clone PAT4B9AT involves several steps:
- Immunization: Mice are immunized with the human Tyr-3/Trp-5 Monooxygenase Activation Protein Gamma to elicit an immune response.
- Hybridoma Production: Spleen cells from the immunized mice are fused with myeloma cells to create hybridoma cells that can produce the desired monoclonal antibody.
- Screening and Selection: Hybridoma cells are screened for the production of antibodies that specifically bind to the Tyr-3/Trp-5 Monooxygenase Activation Protein Gamma. The clone PAT4B9AT is selected for its high specificity and affinity.
- Antibody Purification: The monoclonal antibody is purified from the culture supernatant of the hybridoma cells using techniques such as protein A/G affinity chromatography.
Chemical Reactions Analysis
The Tyr-3/Trp-5 Monooxygenase Activation Protein Gamma is involved in various biochemical pathways and interacts with multiple proteins. Some key interactions and reactions include:
- Phosphorylation: The 14-3-3 proteins, including the gamma isoform, bind to phosphorylated serine/threonine residues on target proteins, modulating their activity and stability.
- Signal Transduction: By interacting with various kinases and phosphatases, the 14-3-3 proteins play a pivotal role in signal transduction pathways, influencing cellular responses to external stimuli.
- Apoptosis Regulation: The 14-3-3 proteins can sequester pro-apoptotic factors in the cytoplasm, preventing them from inducing cell death and thus promoting cell survival.
Applications in Research
The monoclonal antibody clone PAT4B9AT is widely used in research to study the expression, localization, and function of the Tyr-3/Trp-5 Monooxygenase Activation Protein Gamma in human cells. It is utilized in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate the role of this protein in various cellular processes and disease states.
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