YOD1 Human

YOD1 Human Recombinant
Cat. No.
BT18917
Source
Escherichia Coli.
Synonyms
DUBA8, OTUD2, PRO0907, RP11-164O23.1, Ubiquitin thioesterase OTU1, DUBA-8, HIN-7, HsHIN7, OTU domain-containing protein 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

YOD1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 371 amino acids (1-348) and having a molecular mass of 40.7kDa.
YOD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
YOD1, a member of the ovarian tumor (otubain) family, is a highly conserved deubiquitinating Hydrolase. It plays a crucial role in endoplasmic reticulum-associated degradation (ERAD) by removing conjugated ubiquitin from misfolded lumenal proteins. While its exact function in mammalian cells is still being investigated, studies show YOD1 is part of a p97-centered multiprotein complex, suggesting its involvement in misfolded protein dislocation from the endoplasmic reticulum. Inhibiting YOD1's deubiquitinating activity disrupts the dislocation process, as evidenced by the stabilization of several dislocation substrates.
Description
Recombinant human YOD1, expressed in E.coli, is a non-glycosylated polypeptide chain with a molecular weight of 40.7kDa. This single chain protein comprises 371 amino acids, including a 23 amino acid His-tag fused at the N-terminus (amino acids 1-348). Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The YOD1 solution is provided at a concentration of 0.25mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 30% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the YOD1 vial should be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of YOD1 is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
DUBA8, OTUD2, PRO0907, RP11-164O23.1, Ubiquitin thioesterase OTU1, DUBA-8, HIN-7, HsHIN7, OTU domain-containing protein 2.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMFGPAKG RHFGVHPAPG FPGGVSQQAA GTKAGPAGAW PVGSRTDTMW RLRCKAKDGT HVLQGLSSRT RVRELQGQIA AITGIAPGGQ RILVGYPPEC LDLSNGDTIL EDLPIQSGDM LIIEEDQTRP RSSPAFTKRG ASSYVRETLP VLTRTVVPAD NSCLFTSVYY VVEGGVLNPA CAPEMRRLIA QIVASDPDFY SEAILGKTNQ EYCDWIKRDD TWGGAIEISI LSKFYQCEIC VVDTQTVRID RFGEDAGYTK RVLLIYDGIH YDPLQRNFPD PDTPPLTIFS SNDDIVLVQA LELADEARRR RQFTDVNRFT LRCMVCQKGL TGQAEAREHA KETGHTNFGE V.

Product Science Overview

Structure and Expression

YOD1 is composed of 348 amino acids and is characterized by the presence of an OTU domain, which is essential for its deubiquitinating activity . The recombinant form of YOD1 is typically expressed in E. coli and purified using conventional chromatography techniques . The recombinant protein often includes an N-terminal His-tag to facilitate purification and detection.

Function

YOD1’s primary function is to remove ubiquitin moieties from polyubiquitinated proteins, thereby regulating their stability and degradation . This activity is crucial for maintaining cellular homeostasis and preventing the accumulation of misfolded or damaged proteins. YOD1 is a component of a multiprotein complex centered around p97, suggesting its involvement in the dislocation of misfolded proteins from the endoplasmic reticulum .

Role in Disease

Recent studies have highlighted the significance of YOD1 in various diseases, particularly cancer. For instance, YOD1 has been implicated in the progression of triple-negative breast cancer (TNBC) by regulating the stability of CDK1, a key cell cycle protein . Aberrant expression of YOD1 has been associated with poor prognosis in TNBC patients, making it a potential therapeutic target .

Additionally, YOD1 has been identified as a potential prognostic biomarker for pancreatic cancer . Its expression levels correlate with the aggressiveness of pancreatic ductal adenocarcinoma (PDAC), a highly malignant form of pancreatic cancer .

Research and Applications

Recombinant YOD1 is widely used in research to study its biochemical properties and role in various cellular processes. It serves as a valuable tool for investigating the mechanisms of protein ubiquitination and deubiquitination, as well as their implications in disease.

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