WIF1 Mouse

WNT Inhibitory Factor 1 Mouse Recombinant
Cat. No.
BT476
Source
Sf9, Insect cells.
Synonyms
Wnt inhibitory factor 1, WIF-1, Wif1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

WIF1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 359 amino acids (29-379a.a.) and having a molecular mass of 39.4kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
WIF1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
WIF1 is a secreted protein that binds to Wnt proteins and inhibits their signaling activity. Wnt proteins are extracellular signaling molecules that play a crucial role in embryonic development and cancer. WIF1 contains a Wnt inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains. This protein is involved in mesoderm segmentation and is found in various species, including fish, amphibians, and mammals. WIF1 acts as a tumor suppressor, particularly in salivary gland tumors and nonfunctioning pituitary tumors. Downregulation of WIF1 is associated with the development and progression of pleomorphic adenomas.
Description
Recombinant WIF1 protein, expressed in Sf9 insect cells, is a single, glycosylated polypeptide chain with a molecular weight of 39.4 kDa (predicted based on amino acid sequence). Due to glycosylation, the protein migrates at a higher molecular weight of approximately 40-57 kDa on SDS-PAGE. The protein encompasses amino acids 29-379 and includes an 8 amino acid His tag at the C-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless liquid solution, sterile-filtered.
Formulation
WIF1 protein is supplied in a solution at a concentration of 1 mg/ml. The solution is buffered with 20 mM MES (pH 5.5) and contains 1 mM DTT, 1 mM PMSF, and 30% glycerol for protein stability.
Stability
For short-term storage (2-4 weeks), keep the WIF1 protein solution at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage to further enhance protein stability. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of WIF1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Wnt inhibitory factor 1, WIF-1, Wif1.
Source
Sf9, Insect cells.
Amino Acid Sequence
GQPPEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMP AIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVA AFEVNVIVMN SEGNTILRTP QNAIFFKTCQ QAECPGGCRN GGFCNERRVC ECPDGFYGPH
CEKALCIPRC MNGGLCVTPG FCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCELSKC PQPCRNGGKC IGKSKCKCPK GYQGDLCSKP VCEPGCGAHG TCHEPNKCQC REGWHGRHCN KRYGASLMHA PRPAGAGLER HTPSLKKAED RRDPPESNYI WVEHHHHHH.

Product Science Overview

Structure and Function

WIF1 is characterized by the presence of a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains . The protein binds to WNT proteins, preventing them from interacting with their receptors and thereby inhibiting WNT signaling . This inhibition is vital for the proper regulation of cellular processes that are influenced by WNT signaling.

Role in Development

WNT proteins are extracellular signaling molecules that are involved in the control of embryonic development . WIF1, by inhibiting WNT signaling, plays a role in mesoderm segmentation, which is a critical process during the early stages of embryonic development . The protein is found in various species, including fish, amphibians, and mammals .

Expression and Regulation

In mice, WIF1 expression is regulated by androgens and is essential for androgen-dependent prostate development . The protein’s expression is more abundant in male urogenital sinus mesenchyme compared to females, and it overlaps with androgen-responsive genes . This androgen-dependent expression is linked to the formation of prostatic buds during fetal development .

Recombinant WIF1

Recombinant WIF1 proteins are produced using genetic engineering techniques to study their function and potential therapeutic applications. These recombinant proteins are used in various research studies to understand the mechanisms of WNT signaling inhibition and its implications in developmental biology and disease .

Applications in Research

WIF1 has been studied extensively for its role in cancer biology. By inhibiting WNT signaling, WIF1 can suppress tumor growth and progression in various cancers . Additionally, its role in embryonic development makes it a valuable protein for studying developmental disorders and potential therapeutic interventions.

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WIF1 Human
WNT Inhibitory Factor 1 Human Recombinant
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<p><span lang="EN-US">Sf9, Baculovirus cells.</span></p>
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