Sf9, Baculovirus cells.
Greater than 90.0% as determined by SDS-PAGE.
WIF1 Human is a single, glycosylated polypeptide chain containing 360 amino acids (29-379 a.a.) and having a molecular mass of 39.5 kDa
WIF1 is fused to 6 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
ADLGPPQEES LYLWIDAHQA RVLIGFEEDI LIVSEGKMAP FTHDFRKAQQ RMPAIPVNIH SMNFTWQAAG QAEYFYEFLS LRSLDKGIMA DPTVNVPLLG TVPHKASVVQ VGFPCLGKQD GVAAFEVDVI VMNSEGNTIL QTPQNAIFFK TCQQAECPGG CRNGGFCNER RICECPDGFH GPHCEKALCT PRCMNGGLCV TPGFCICPPG FYGVNCDKAN CSTTCFNGGT CFYPGKCICP PGLEGEQCEI SKCPQPCRNG GKCIGKSKCK CSKGYQGDLC SKPVCEPGCG AHGTCHEPNK CQCQEGWHGR HCNKRYEASL IHALRPAGAQ LRQHTPSLKK AEERRDPPES NYIWHHHHHH
WIF1 contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains . The WIF domain is responsible for binding to WNT proteins, preventing them from triggering signaling pathways that are crucial for embryonic development and other cellular processes . This protein is found in various species, including fish, amphibians, and mammals .
WNT proteins are extracellular signaling molecules involved in the control of embryonic development. By inhibiting WNT proteins, WIF1 plays a significant role in processes such as mesoderm segmentation . Additionally, WIF1 functions as a tumor suppressor gene and has been found to be epigenetically silenced in various cancers . This makes it a potential target for cancer therapy and research.
Recombinant human WIF1 is typically produced using expression systems such as HEK293 cells . The protein is expressed with a His tag to facilitate purification. The recombinant protein is then purified using techniques like SDS-PAGE to ensure high purity levels (typically above 95%) . The lyophilized protein can be stored at -20°C to -80°C for long-term stability .
WIF1 interacts with WNT proteins through its WIF domain, effectively inhibiting the WNT signaling pathway . This interaction is crucial for maintaining the balance of cellular processes regulated by WNT signaling. The inhibition of WNT signaling by WIF1 can be analyzed using various biochemical and molecular biology techniques, including binding assays and signal transduction studies .