VPS29 Mouse

VPS29 is a late Golgi transmembrane protein that acts as a sorting receptor for soluble vacuolar hydrolases. It is involved in the retrograde transport of these hydrolases from the prevacuolar endosome back to the Golgi apparatus . The protein is part of the retromer complex, which also includes VPS26 and VPS35. Together, these proteins form a stable core that is essential for the formation of the retromer coat, which facilitates the sorting and transport of cargo proteins.

Recombinant Mouse VPS29 is a version of the protein that has been engineered for research purposes. It is typically expressed in E. coli and purified using conventional chromatography techniques . The recombinant protein often includes a His-tag at the N-terminus to facilitate purification and detection. The amino acid sequence of the recombinant mouse VPS29 corresponds to the first 182 amino acids of the native protein .

Recombinant VPS29 is used extensively in research to study the mechanisms of protein sorting and trafficking within cells. It is particularly valuable in understanding the role of the retromer complex in various cellular processes, including receptor recycling, lysosome biogenesis, and autophagy. Studies have shown that VPS29 is essential for the recycling of numerous cell-surface cargoes from endosomes .

Mutations or dysfunctions in VPS29 and other components of the retromer complex have been linked to various diseases, including neurodegenerative disorders like Alzheimer’s disease. Research involving recombinant VPS29 helps in elucidating the molecular mechanisms underlying these diseases and can potentially lead to the development of therapeutic strategies.

Recombinant Mouse VPS29 is typically stored in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 10% glycerol, and 0.1 M NaCl . It should be stored at 4°C for short-term use and at -20°C for long-term storage to avoid freeze-thaw cycles that could degrade the protein.