VPS29 Human

Vacuolar Protein Sorting 29 Human Recombinant
Cat. No.
BT24856
Source
E.coli.
Synonyms
Vacuolar protein sorting 29 yeast homolog (S. cerevisiae), DC15, PEP11, DC7, retromer protein, x 007 protein, EC 3.1.3.3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

VPS29 Human Recombinant produced in E. coli is a single polypeptide chain containing 207 amino acids (1-182) and having a molecular mass of 23.2kDa.
VPS29 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
VPS29 is a member of the vacuolar protein sorting (VPS) gene family. These genes encode proteins essential for the efficient trafficking of vacuolar hydrolases. Disruption of VPS29 function can impair this process. As a late Golgi transmembrane protein, VPS29 acts as a sorting receptor, retrieving soluble vacuolar hydrolases from the prevacuolar endosome and returning them to the Golgi. Additionally, VPS29 contributes to the formation of the inner shell of the retromer coat, which is crucial for retrograde vesicles budding from the prevacuolar compartment.
Description
Recombinant human VPS29, expressed in E. coli, is a single polypeptide chain with a molecular weight of 23.2 kDa. This protein comprises 207 amino acids, including a 25 amino acid His-tag fused at the N-terminus (amino acids 1-182). Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile filtered solution.
Formulation
The VPS29 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 40% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Vacuolar protein sorting 29 yeast homolog (S. cerevisiae), DC15, PEP11, DC7, retromer protein, x 007 protein, EC 3.1.3.3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMLVLV LGDLHIPHRC NSLPAKFKKL LVPGKIQHIL CTGNLCTKES YDYLKTLAGD VHIVRGDFDE NLNYPEQKVV TVGQFKIGLI HGHQVIPWGD MASLALLQRQ FDVDILISGH THKFEAFEHE NKFYINPGSA TGAYNALETN IIPSFVLMDI QASTVVTYVY QLIGDDVKVE RIEYKKP

Product Science Overview

Structure and Function

VPS29 is part of a larger multimeric complex known as the retromer complex. This complex is essential for the proper sorting and trafficking of proteins within the cell. The retromer complex typically consists of VPS26, VPS29, and VPS35, along with sorting nexins (SNX1 and SNX2) which are involved in the retrograde transport of proteins from endosomes to the trans-Golgi network .

The crystal structure of human VPS29 reveals that it participates in the formation of the retromer complex by binding to other components such as VPS26 and VPS35. This interaction is crucial for the stability and function of the retromer complex .

Biological Role

VPS29 is involved in several key biological processes, including:

  • Protein Transport: It facilitates the transport of proteins from endosomes back to the trans-Golgi network, ensuring that proteins are correctly sorted and delivered to their intended destinations .
  • Regulation of Autophagy: VPS29 plays a role in the regulation of autophagy, a process that helps maintain cellular homeostasis by degrading and recycling cellular components .
  • Wnt Signaling Pathway: It is also involved in the Wnt signaling pathway, which is important for cell development and differentiation .
Clinical Significance

Mutations or dysfunctions in the VPS29 gene can lead to disruptions in protein trafficking, which may result in various cellular and physiological abnormalities. Understanding the structure and function of VPS29 is therefore crucial for developing potential therapeutic strategies for diseases related to protein sorting and trafficking.

Research and Applications

Recombinant human VPS29 is widely used in research to study its role in the retromer complex and its interactions with other proteins. This research is essential for elucidating the molecular mechanisms underlying protein sorting and trafficking within cells.

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