Vaspin Antibody
Description
Product Specs
Product Science Overview
Vaspin, also known as Visceral Adipose Tissue-Derived Serine Protease Inhibitor or SERPINA12, is a member of the serine protease inhibitor (serpin) family. It was first discovered in 2005 in the visceral adipose tissue of Otsuka Long-Evans Tokushima Fatty (OLETF) rats, which are a model for obesity and type 2 diabetes. Vaspin expression was found to peak when the rats’ obesity and insulin plasma levels were at their highest .
In humans, vaspin is encoded by the SERPINA12 gene located on chromosome 14. The protein consists of 414 amino acids and has a molecular weight of approximately 47 kDa . Vaspin is primarily expressed in visceral and subcutaneous adipose tissues but is also found in other tissues such as the liver, pancreas, and skin .
Vaspin plays a crucial role in modulating insulin sensitivity and glucose metabolism. It acts as an insulin-sensitizing adipokine, which means it helps improve the body’s response to insulin. This function is particularly significant in the context of obesity and type 2 diabetes, where insulin resistance is a common issue . Vaspin achieves this by inhibiting proteases that degrade insulin receptors, thereby enhancing insulin signaling pathways .
Additionally, vaspin has anti-inflammatory properties. It suppresses the inflammatory phenotypes in macrophages and vascular smooth muscle cells, which are often involved in the development of atherosclerosis . This makes vaspin a potential therapeutic target for metabolic and cardiovascular diseases.
Mouse anti-human vaspin antibodies are monoclonal antibodies produced by immunizing mice with human vaspin protein. These antibodies are highly specific to human vaspin and are used extensively in research to study the protein’s function and role in various diseases.
Production and Characteristics
The production of mouse anti-human vaspin antibodies involves the hybridization of mouse myeloma cells with spleen cells from mice immunized with recombinant human vaspin. The resulting hybridoma cells are capable of producing large quantities of monoclonal antibodies .
These antibodies are typically purified using protein G affinity chromatography and are available in various formats, including unconjugated and conjugated forms. They are used in a range of applications such as Western blotting, ELISA, and immunohistochemistry .
Applications in Research
Mouse anti-human vaspin antibodies are invaluable tools in biomedical research. They are used to:
- Detect and quantify vaspin levels in biological samples through techniques like ELISA and Western blotting.
- Study the distribution and localization of vaspin in tissues using immunohistochemistry.
- Investigate the role of vaspin in metabolic and cardiovascular diseases by blocking or enhancing its activity in experimental models .
