VAMP4 Human
Vesicle-associated membrane protein 4 Human Recombinant
Cat. No.
BT5725
Source
Escherichia Coli.
Synonyms
Vesicle-associated membrane protein 4, VAMP-4, VAMP4, VAMP24.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
VAMP4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 123 amino acids (1-115 a.a.) and having a molecular mass of 14.5kDa.
VAMP4 is fused to an 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.
VAMP4 is fused to an 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
VAMP4, a member of the synaptobrevin family, plays a crucial role in vesicle docking and fusion with the cell membrane. Primarily located in the trans-Golgi network, VAMP4 is believed to be involved in the transport of molecules from the trans-Golgi network to endosomes. It participates in a pathway responsible for removing an inhibitor, likely synaptotagmin-4, which regulates calcium-triggered exocytosis during secretory granule maturation. The core components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane include synaptobrevins, syntaxins, and SNAP25.
Description
Recombinant human VAMP4, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 123 amino acids (amino acids 1-115) with a molecular weight of 14.5 kDa. A His tag is fused to the C-terminus of VAMP4. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The VAMP4 protein is supplied in a solution containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Vesicle-associated membrane protein 4, VAMP-4, VAMP4, VAMP24.
Source
Escherichia Coli.
Amino Acid Sequence
MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH.
Product Science Overview
Introduction
Vesicle-associated membrane protein 4 (VAMP4) is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. These proteins are integral to the process of vesicle-mediated transport, particularly in the context of synaptic vesicle docking and fusion with the presynaptic membrane .
Gene and Protein Structure
Function
VAMP4 plays a crucial role in the trans-Golgi network-to-endosome transport pathway. It is involved in the removal of an inhibitor (likely synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules . This function is critical for the remodeling of the secretory response of granules, making VAMP4 a marker for this sorting pathway .
Pathways and Interactions
Clinical Significance
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