UPP1 Salmonella
Description
Product Specs
30 U/mg protein.
Product Science Overview
Salmonella Typhimurium is a gram-negative bacterium that is commonly associated with foodborne illnesses. It is a member of the Enterobacteriaceae family and is known for its ability to cause gastroenteritis in humans and animals. The bacterium has been widely used in research due to its well-characterized genetics and its ability to be manipulated for various experimental purposes.
Recombinant technology involves the insertion of a gene of interest into a host organism to produce a desired protein. In the case of recombinant uridine phosphorylase from Salmonella Typhimurium, the gene encoding UPase is cloned into a suitable expression vector and introduced into a host, such as Escherichia coli. This allows for the production of large quantities of the enzyme, which can then be purified and studied.
The recombinant form of uridine phosphorylase from Salmonella Typhimurium has several important applications:
- Biocatalysis: UPase can be used in the synthesis of nucleoside analogs, which are important in the development of antiviral and anticancer drugs.
- Metabolic Engineering: The enzyme can be used to engineer metabolic pathways in microorganisms to enhance the production of valuable metabolites.
- Structural Studies: Recombinant UPase provides a model system for studying the structure and function of pyrimidine salvage enzymes.
Research has shown that different strains of Salmonella Typhimurium can vary in their virulence and immunogenicity. For example, studies have compared the immunological responses of mice to different recombinant strains expressing various antigens . These studies help in understanding the optimal strategies for vaccine development and the role of inherent virulence in the efficacy of live attenuated vaccines.
