UQCRC1 Human

Ubiquinol-Cytochrome C Reductase Core Protein I Human Recombinant
Cat. No.
BT21142
Source
Escherichia Coli.
Synonyms
D3S3191, QCR1, UQCR1, Cytochrome b-c1 complex subunit 1, mitochondrial, Complex III subunit 1, Core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

UQCRC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 471 amino acids (35-480 a.a) and having a molecular mass of 51.8kDa.UQCRC1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ubiquinol-Cytochrome C Reductase Core Protein I (UQCRC1), also known as QCR1, belongs to the peptidase M16 family and the UQCRC1/QCR1 subfamily. This protein is an integral part of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), a crucial component of the mitochondrial respiratory chain. UQCRC1 plays a vital role in the formation of the complex between cytochromes c and c1.
Description
This product consists of the recombinant human UQCRC1 protein, produced in E. coli. It is a single, non-glycosylated polypeptide chain composed of 471 amino acids (specifically, amino acids 35-480). The protein has a molecular weight of 51.8kDa. A 25 amino acid His-tag is fused to the N-terminus of the UQCRC1 protein. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
The product appears as a clear, sterile-filtered solution.
Formulation
The UQCRC1 protein solution is provided at a concentration of 1mg/ml. The solution is buffered with 20mM Tris-HCl at pH 8.0 and supplemented with 0.4M UREA and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For extended storage, freezing at -20°C is recommended. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised. It's important to avoid repeated freezing and thawing of the product.
Purity
The purity of the UQCRC1 protein is determined by SDS-PAGE analysis and exceeds 85.0%.
Synonyms
D3S3191, QCR1, UQCR1, Cytochrome b-c1 complex subunit 1, mitochondrial, Complex III subunit 1, Core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFTATFA QALQFVPETQ VSLLDNGLRV ASEQSSQPTC TVGVWIDVGS RFETEKNNGA GYFLEHLAFK GTKNRPGSAL EKEVESMGAH LNAYSTREHT AYYIKALSKD LPKAVELLGD IVQNCSLEDS QIEKERDVIL REMQENDASM RDVVFNYLHA TAFQGTPLAQ AVEGPSENVR KLSRADLTEY LSTHYKAPRM VLAAAGGVEH QQLLDLAQKH LGGIPWTYAE DAVPTLTPCR FTGSEIRHRD DALPFAHVAI AVEGPGWASP DNVALQVANA IIGHYDCTYG GGVHLSSPLA SGAVANKLCQ SFQTFSICYA ETGLLGAHFV CDRMKIDDMM FVLQGQWMRL CTSATESEVA RGKNILRNAL VSHLDGTTPV CEDIGRSLLT YGRRIPLAEW ESRIAEVDAS VVREICSKYI YDQCPAVAGY GPIEQLPDYN RIRSGMFWLR F.

Product Science Overview

Introduction

Ubiquinol-Cytochrome C Reductase Core Protein I (UQCRC1) is a crucial component of the mitochondrial electron transport chain, specifically within Complex III, also known as the cytochrome bc1 complex . This protein plays a vital role in cellular respiration and energy production in eukaryotic cells.

Gene and Protein Structure

The UQCRC1 gene is located on chromosome 3 in humans and encodes a protein that is a subunit of the ubiquinol-cytochrome c reductase complex . This complex is composed of multiple subunits, including both mitochondrially and nuclear-encoded proteins . The UQCRC1 protein itself is highly conserved across species, indicating its essential function in cellular metabolism .

Function

UQCRC1 is involved in the electron transport chain, where it facilitates the transfer of electrons from ubiquinol to cytochrome c . This process is coupled with the translocation of protons across the mitochondrial inner membrane, contributing to the generation of the electrochemical gradient used by ATP synthase to produce ATP . This makes UQCRC1 a key player in oxidative phosphorylation and energy production in cells.

Clinical Significance

Mutations or dysfunctions in the UQCRC1 gene have been associated with various diseases, including mitochondrial disorders and neurodegenerative diseases such as Parkinsonism and Alzheimer’s disease . Research has shown that UQCRC1 can act as a biomarker for these conditions, highlighting its importance in medical diagnostics and potential therapeutic targets .

Recombinant UQCRC1

Recombinant UQCRC1 is produced using genetic engineering techniques, where the UQCRC1 gene is cloned and expressed in a suitable host system, such as bacteria or yeast . This allows for the production of large quantities of the protein for research and therapeutic purposes. Recombinant UQCRC1 is used in various studies to understand its function, structure, and role in diseases, as well as in the development of potential treatments .

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