UMOD Porcine
Uromodulin Porcine
Cat. No.
BT20747
Source
Porcine Urine.
Synonyms
Tamm-Horsfall urinary glycoprotein, THP, FJHN, HNFJ, THGP, MCKD2, ADMCKD2, UMOD, Uromodulin.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Porcine Uromodulin is a 97kDa glycoprotein which is produced in the thick ascending limb of Henle´s loop and early distal convoluted tubules of the nephron.
Product Specs
Introduction
Uromodulin, the most abundant protein in normal urine, is secreted after proteolytic cleavage of its glycosyl phosphatidylinosital-anchored counterpart on the luminal surface of the loop of Henle. It acts as a natural inhibitor of calcium crystallization in renal fluids and protects against urinary tract infections caused by uropathogenic bacteria. Defects in Uromodulin expression are linked to autosomal dominant renal disorders like medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN), characterized by early-onset hyperuricemia, gout, and progressive renal failure. Although multiple transcript variants might exist, only two have been fully characterized. UMOD regulates circulating cytokine activity by binding to interleukin-1 (IL-1), IL-2, and tumor necrosis factor (TNF) with high affinity.
Description
Porcine Uromodulin, a 97kDa glycoprotein, is synthesized in the thick ascending limb of Henle's loop and the early distal convoluted tubules within the nephron.
Physical Appearance
White, lyophilized powder that has been filtered for purity.
Formulation
The Uromodulin protein was lyophilized from a 0.4 µm filtered solution with a concentration of 0.5 mg/mL in deionized water.
Solubility
To create a working stock solution of approximately 0.5 mg/mL, add deionized water to the lyophilized pellet and allow it to dissolve completely. Please note that this product is not sterile. Filter it through a sterile filter before using it in cell culture.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freezing and thawing. The reconstituted protein remains stable at 4°C for a limited period and shows no changes after two weeks at this temperature.
Synonyms
Tamm-Horsfall urinary glycoprotein, THP, FJHN, HNFJ, THGP, MCKD2, ADMCKD2, UMOD, Uromodulin.
Source
Porcine Urine.
Product Science Overview
Structure and Production
Uromodulin is a 97kDa glycoprotein . In its porcine form, it is derived from porcine urine and is typically available as a lyophilized (freeze-dried) powder . The protein is produced through proteolytic cleavage of its glycosyl phosphatidylinositol-anchored counterpart situated on the luminal cell surface of the loop of Henle .
Functions
Uromodulin plays several critical roles in renal physiology and systemic health:
- Inhibition of Calcium Crystallization: It acts as a constitutive inhibitor of calcium crystallization in renal fluids, which helps prevent kidney stone formation .
- Protection Against Urinary Tract Infections: Uromodulin provides a defense mechanism against urinary tract infections by preventing the adhesion of uropathogenic bacteria to the urinary tract .
- Regulation of Cytokine Activity: It binds to cytokines such as IL-1, IL-2, and TNF with high affinity, thereby regulating their circulating activity .
Clinical Significance
Defects in uromodulin expression are associated with several autosomal dominant renal disorders, including:
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