UMOD Canine
Uromodulin Canine
Cat. No.
BT20415
Source
Canine Urine.
Synonyms
Tamm-Horsfall urinary glycoprotein, THP, FJHN, HNFJ, THGP, MCKD2, ADMCKD2, UMOD, Uromodulin.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
UMOD is an 85-kDa glycoprotein which is produced in the thick ascending limb of Henle´s loop and early distal convoluted tubules of the nephron.
Product Specs
Introduction
Canine uromodulin, similar to its human counterpart, is the most abundant protein found in normal urine. It is released into the urine after the ectodomain of its glycosyl phosphatidylinosital-anchored form, present on the luminal surface of the loop of Henle cells, undergoes proteolytic cleavage. Uromodulin acts as a natural inhibitor of calcium crystallization in the renal fluids. Its presence in urine provides a defense mechanism against urinary tract infections caused by uropathogenic bacteria. Although not yet fully elucidated in canines, studies in other species suggest that defects in UMOD expression may be linked to renal disorders characterized by hyperuricemia and potential renal complications. Further research is needed to establish a definitive connection between UMOD and specific canine renal diseases.
Description
Canine UMOD, an 85-kDa glycoprotein, is primarily synthesized in the thick ascending limb of Henle's loop and the early distal convoluted tubules within the nephron.
Physical Appearance
Filtered White lyophilized (freeze-dried) powder.
Formulation
The canine UMOD protein was lyophilized from a 0.4µm filtered solution at a concentration of 0.1mg/ml in deionized water.
Solubility
To create a working stock solution of about 0.5mg/mL, add deionized water to the lyophilized pellet and allow it to dissolve completely. Please note that this product is not sterile. Before using it in cell culture, it is essential to filter the solution through an appropriate sterile filter.
Stability
Lyophilized canine UMOD, while stable at room temperature for up to 3 weeks, should ideally be stored in a desiccated state below -18°C. After reconstitution, UMOD should be stored at 4°C for 2-7 days. For long-term storage, freezing below -18°C is recommended. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles to maintain product integrity.
Synonyms
Tamm-Horsfall urinary glycoprotein, THP, FJHN, HNFJ, THGP, MCKD2, ADMCKD2, UMOD, Uromodulin.
Source
Canine Urine.
Amino Acid Sequence
RSCSECHSNA TCMEDGMVTT CSCLVGFTGS GFECVDLDEC AIPGAHNCSE GSSCMNTLGS YLCTCPDGFR LTPGLGCIDV DECSEPGLSR CHALATCINN KGNYSCVCPA GYRGDGQHCE CSPGSCGPGL DCVPVGDALV CADPCQEHRI LDEYWRSTEY GAGYTCDVGL NGWYRFTGPG GVRLAETCVP VLHCNTAAPM WLNGTHPTRD QGIVNRTACA HWRGHCCLWD ASIQVKACAG GYYVYNLTET PECYLAYCTD PTSVLGTCEE CSVEEDCKSH DGMWSCQCKQ DFNVTDLFLL DRLECRPNDI KVSLSKCQLK SLGFEKVFMY LRDSQCSGFN ERGDRDWVSV VTPARDGPCG TVMVRNETHA TYSNTLYLAD EIVIRDRNIK INFECSYPLD MKVSLETSLQ PIVSSLNISV GGTGMFTVRM ALFQTPDYTQ PYQGSSVTLT TEAFLYVGTM LDGGDLSRFA LLMTNCYATP SSNATDPLKY FIIQDRCPRT TDSTIQVVEN GESPQGRFSV QMFRFAGNYD LVYLHCEVYL CDIINEKCKP TCSGTRFRSG GIIDQSRVLN LGPITRKNVQ AVVSRAASS.
Product Science Overview
Historical Context
Biological Function
Uromodulin plays several critical roles in the body:
- Immune Response: It helps in the defense against urinary tract infections by binding to pathogens and preventing their adhesion to the urinary tract .
- Regulation of Mineral Balance: Uromodulin is involved in the regulation of calcium and magnesium reabsorption in the kidneys .
- Protection Against Kidney Stones: It inhibits the aggregation of calcium crystals, thereby reducing the risk of kidney stone formation .
Research in Canines
Recent studies have focused on the levels of uromodulin in both serum and urine of dogs, particularly those with CKD. These studies have shown that:
- Serum Uromodulin: The concentration of uromodulin in the serum is significantly lower in dogs with CKD compared to healthy dogs . This makes it a potential biomarker for early detection of kidney disease.
- Urinary Uromodulin: The levels of uromodulin in urine, when indexed to urinary creatinine, are also reduced in dogs with CKD . This reduction is more pronounced in certain breeds, such as Belgian shepherds, compared to others like German shepherds .
Diagnostic Potential
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