ULBP3 Human

UL16 Binding Protein 3 Human Recombinant
Cat. No.
BT24081
Source
Escherichia Coli.
Synonyms
UL16 Binding Protein 3, Retinoic Acid Early Transcript 1N, ALCAN-Gamma, NKG2DL3, N2DL-3, RAET1N, UL16-Binding Protein 3, NKG2D Ligand 3, N2DL3, ULBP3.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ULBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Asp30-Gly217) containing 198 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 23.25kDa.

Product Specs

Introduction
UL16 Binding Protein 3 (ULBP3) is a cell surface protein that acts as a ligand for the NKG2D receptor, a key activating receptor found on Natural Killer (NK) cells. ULBPs play a critical role in the immune system's response to cellular stress and infection by activating various signaling pathways that lead to the production of cytokines and chemokines, which are crucial for immune cell communication and recruitment. Notably, ULBP3, along with ULBP1 and ULBP2, binds to the KLRK1/NKG2D receptor, initiating a cascade of events that include calcium mobilization within the cell and the activation of essential signaling pathways such as JAK2, STAT5, ERK, and PI3K kinase/Akt. These pathways contribute to the overall immune response against stressed or infected cells. Interestingly, ULBP3 exhibits a lower affinity for the KLRK1/NKG2D receptor compared to ULBP1 and ULBP2, resulting in weaker signaling responses.
Description
ULBP3 Human Recombinant is a laboratory-produced protein designed to mimic the structure and function of naturally occurring human ULBP3. It is expressed in E. coli bacteria and undergoes a purification process to ensure its quality and purity. This recombinant protein is a single polypeptide chain consisting of 198 amino acids, representing the primary structure of ULBP3. Importantly, it lacks any post-translational modifications like glycosylation, making it a simplified version of the naturally occurring protein. For ease of handling and storage, it is provided in a lyophilized (freeze-dried) powder form.
Physical Appearance
White, solid substance in a powdered form.
Formulation
To ensure stability and prevent degradation, the ULBP3 protein is carefully prepared before lyophilization. It is first filtered through a 0.4-micrometer filter to remove any large particles or impurities. The protein is then lyophilized in a buffer solution containing 20mM Tris, 50mM NaCl, and 0.1% v/v amisoft CS-22, with a pH adjusted to 7.5. This specific formulation helps to maintain the protein's integrity and stability during storage.
Solubility
To prepare a working solution, add deionized water to the lyophilized ULBP3 powder to achieve a concentration of approximately 0.5mg/ml. Allow sufficient time for the powder to dissolve completely. It is important to note that the provided ULBP3 is not sterile. Before using it in cell culture experiments, it is crucial to sterilize the solution by filtering it through an appropriate sterile filter. This step ensures that no bacteria or other contaminants are introduced into the cell culture, which could compromise the results of experiments.
Stability
To maintain the long-term stability of the lyophilized ULBP3 protein, store it at -20°C. Once the protein is reconstituted, it is recommended to divide it into smaller portions (aliquots) and store them separately. This practice helps minimize repeated freeze-thaw cycles, which can potentially damage the protein's structure and activity. After reconstitution, the protein can be stored at 4°C for a short period, typically up to two weeks, without significant loss of activity.
Purity
The purity of the ULBP3 protein is determined using SDS-PAGE, a widely used technique for analyzing protein purity. Our analysis shows that the purity of ULBP3 is greater than 90%, indicating a high-quality protein suitable for various research applications.
Synonyms
UL16 Binding Protein 3, Retinoic Acid Early Transcript 1N, ALCAN-Gamma, NKG2DL3, N2DL-3, RAET1N, UL16-Binding Protein 3, NKG2D Ligand 3, N2DL3, ULBP3.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASDAHSLWYNFT IIHLPRHGQQ WCEVQSQVDQ KNFLSYDCGS DKVLSMGHLE EQLYATDAWG KQLEMLREVG QRLRLELADT ELEDFTPSGP LTLQVRMSCE CEADGYIRGS WQFSFDGRKF LLFDSNNRKW TVVHAGARRM KEKWEKDSGL TTFFKMVSMR DCKSWLRDFL MHRKKRLEPT APPTMAPG.

Product Science Overview

Introduction

UL16 Binding Protein 3 (ULBP3) is a member of the UL16-binding proteins (ULBPs), which are part of the extended MHC class I superfamily. These proteins are known for their role in the immune response, particularly in the activation of natural killer (NK) cells. ULBP3, along with other ULBPs, serves as a ligand for the NKG2D receptor, a crucial activating receptor on NK cells, CD8+ T cells, and some γδ T cells .

Gene and Protein Structure

The ULBP3 gene is located on chromosome 6 and is part of a cluster of ten related genes, six of which encode potentially functional glycoproteins . Unlike classical MHC class I molecules, ULBPs do not contain the alpha 3 domain and lack a transmembrane domain . The protein structure includes α1 and α2 domains, which are essential for binding to the NKG2D receptor .

Expression and Tissue Distribution

ULBP3 is expressed on the surface of many tumor cells and can also be found in a soluble form . Its expression is often upregulated in response to cellular stress, infection, or transformation, making it a potential target for therapeutic interventions .

Biological Function

The primary function of ULBP3 is to bind and activate the NKG2D receptor on NK cells and certain T cells . This interaction triggers several signal transduction pathways, including the JAK2, STAT5, and ERK pathways, leading to the activation of cytotoxic responses against infected or malignant cells . By engaging the NKG2D receptor, ULBP3 plays a critical role in the immune surveillance and elimination of abnormal cells .

Recombinant ULBP3

Recombinant ULBP3 is produced using various expression systems to study its function and potential therapeutic applications. The recombinant form retains the ability to bind the NKG2D receptor and can be used in research to understand its role in immune responses and its potential as a therapeutic target .

Clinical Implications

Given its role in activating NK cells and its expression on tumor cells, ULBP3 is being investigated as a potential target for cancer immunotherapy . Therapeutic strategies may include enhancing ULBP3 expression on tumor cells to boost immune recognition or using soluble forms of ULBP3 to modulate immune responses .

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