ULBP3 Human, Sf9
Sf9, Baculovirus cells.
UL16 Binding Protein 3, Retinoic Acid Early Transcript 1N, ALCAN-Gamma, NKG2DL3, N2DL-3, RAET1N, UL16-Binding Protein 3, NKG2D Ligand 3, N2DL3, ULBP3
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
ULBP3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 430 amino acids (30-217a.a) and having a molecular mass of 49.3kDa.
ULBP3 is fused to a 239 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
UL16 Binding Protein 3 (ULBP3), also known as NKG2DL3, is a cell surface protein that acts as a ligand for the NKG2D receptor found on natural killer (NK) cells and some T cells. ULBP3 is part of the ULBP family, which plays a crucial role in the immune system's recognition and elimination of infected or cancerous cells. When ULBP3 binds to NKG2D, it triggers the activation of these immune cells, leading to the release of cytotoxic molecules and the initiation of cell death in the target cell. ULBP3's interaction with NKG2D is essential for immune surveillance and control of tumor growth.
Recombinant human ULBP3 protein has been produced using Sf9 insect cells infected with a baculovirus expression system. This method results in a glycosylated polypeptide chain containing 430 amino acids, encompassing residues 30 to 217a.a of the ULBP3 protein. The recombinant ULBP3 has a molecular weight of approximately 49.3 kDa. To facilitate purification and detection, a 239 amino acid hIgG-His-Tag is fused to the C-terminus of the protein. The protein is purified to a high degree using proprietary chromatographic techniques.
The ULBP3 protein solution appears as a clear and colorless liquid after sterilization by filtration.
The ULBP3 protein is supplied as a solution with a concentration of 1 mg/ml. The solution is formulated in a Phosphate-Buffered Saline (PBS) buffer at pH 7.4 and contains 10% glycerol as a stabilizing agent.
To ensure product stability, store the ULBP3 protein solution at 4°C if it will be used within 2-4 weeks. For longer-term storage, freeze the solution at -20°C. To maximize stability during long-term storage, it is recommended to add a carrier protein, such as HSA or BSA, to a final concentration of 0.1%. Repeated freezing and thawing of the protein solution should be avoided to maintain optimal product quality.
The purity of the ULBP3 protein is determined by SDS-PAGE analysis and is consistently found to be greater than 95%.
UL16 Binding Protein 3, Retinoic Acid Early Transcript 1N, ALCAN-Gamma, NKG2DL3, N2DL-3, RAET1N, UL16-Binding Protein 3, NKG2D Ligand 3, N2DL3, ULBP3
Sf9, Baculovirus cells.
ADPDAHSLWY NFTIIHLPRH GQQWCEVQSQ VDQKNFLSYD CGSDKVLSMG HLEEQLYATD AWGKQLEMLR EVGQRLRLEL ADTELEDFTP SGPLTLQVRM SCECEADGYI RGSWQFSFDG RKFLLFDSNN RKWTVVHAGA RRMKEKWEKD SGLTTFFKMV SMRDCKSWLR DFLMHRKKRL EPTAPPTMAP GLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH
Product Science Overview
ULBP3 is a glycoprotein that is produced in Sf9 Baculovirus cells. The recombinant form of ULBP3 is a single, glycosylated polypeptide chain containing 430 amino acids, with a molecular mass of approximately 49.3 kDa . This recombinant protein is fused to a 239 amino acid hIgG-His-Tag at the C-terminus, which aids in its purification through proprietary chromatographic techniques .
ULBP3, along with other ULBPs, binds to the KLRK1/NKG2D receptor on NK cells. This interaction triggers various signaling pathways, including the JAK2, STAT5, ERK, and PI3K/Akt pathways . These pathways are essential for the mobilization of calcium and the activation of NK cells, leading to the production of cytokines and chemokines .
Compared to other ULBPs, ULBP3 has a lower affinity for the NKG2D receptor and stimulates weaker signaling responses . Despite this, it still plays a significant role in the immune response by enhancing the cytotoxic activity of NK cells and promoting the production of inflammatory cytokines .
The recombinant form of ULBP3 produced in Sf9 cells is widely used in laboratory research. It is particularly valuable for studying the mechanisms of NK cell activation and the role of NKG2D ligands in immune responses . Researchers use this protein to investigate the signaling pathways involved in NK cell activation and to develop potential therapeutic strategies for enhancing immune responses against tumors and viral infections .
ULBP3 (Human Recombinant, Sf9) is typically supplied as a sterile, filtered, colorless solution. It is formulated with 10% glycerol and phosphate-buffered saline (pH 7.4) to ensure stability . For short-term storage, it can be kept at 4°C for up to 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .
