Introduction
UBL5, unlike ubiquitin which degrades proteins, is thought to be a reversible modulator of protein function. It influences a crucial nuclear phase that enhances the response to the threat of mitochondrial protein misfolding.
Description
Recombinant Human UBL5, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 93 amino acids (1-73a.a) and has a molecular mass of 10.7 kDa. This UBL3 protein is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile filtered solution.
Formulation
UBL5 protein solution at a concentration of 1mg/ml, in a buffer of 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.1M NaCl, and 2mM DTT.
Stability
While UBL5 can remain stable at 4°C for up to one week, it is recommended to store it below -18°C for long-term storage. It is important to avoid repeated freeze-thaw cycles.
Purity
Purity levels are greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Ubiquitin-like protein 5, HUB1, FLJ46917, MGC131795.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MIEVVCNDRL GKKVRVKCNT DDTIGDLKKL IAAQTGTRWN KIVLKKWYTI FKDHVSLGDY EIHDGMNLEL YYQ