UBL4A Human

Ubiquitin-Like 4A Human Recombinant
Cat. No.
BT22696
Source
Escherichia Coli.
Synonyms
Ubiquitin-like protein 4A, Ubiquitin-like protein GDX, UBL4A, DXS254E, GDX, UBL4, G6PD, GET5, MDY2, TMA24, DX254E.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

UBL4A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (1-157 a.a.) and having a molecular mass of 18.8kDa.
UBL4A is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ubiquitin-Like 4A (UBL4A) is a protein containing a ubiquitin-like domain. It is part of the BAT3 complex, a multiprotein complex involved in delivering tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. This process is crucial as it ensures proteins are correctly localized within the cell. UBL4A, through its ubiquitin-like domain, is involved in post-translational modification, a vital regulatory mechanism controlling protein degradation and essential cellular processes. Notably, in mammals, during late stages of sperm development (spermiogenesis), transcription ceases due to the compaction of the DNA. This makes post-transcriptional regulation, in which UBL4A plays a role, even more critical for proper sperm cell formation.
Description
Recombinant human UBL4A protein was produced in E. coli bacteria. This protein is a single chain with 165 amino acids (specifically amino acids 1 to 157 of the human UBL4A sequence) and a molecular weight of 18.8 kDa. It is not glycosylated, meaning it does not have sugars attached. For purification and detection purposes, the UBL4A protein has an 8 amino acid His-tag attached to its C-terminus. The protein was purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Clear and colorless solution that has been sterilized by filtration.
Formulation
The UBL4A protein solution has a concentration of 1 mg/ml and is prepared in a buffer containing 20mM Tris-HCl (pH 8.0), 1mM DTT, 10% glycerol, and 100mM NaCl. This specific formulation helps to maintain the protein's stability and activity.
Stability
To ensure the stability of the UBL4A protein, store the vial at 4°C if you plan to use it within 2-4 weeks. For longer storage, freeze the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. Repeated freezing and thawing of the protein should be avoided to prevent degradation.
Purity
The purity of the UBL4A protein is greater than 95%, as assessed by SDS-PAGE, a widely used technique to separate and analyze proteins based on their size.
Synonyms
Ubiquitin-like protein 4A, Ubiquitin-like protein GDX, UBL4A, DXS254E, GDX, UBL4, G6PD, GET5, MDY2, TMA24, DX254E.
Source
Escherichia Coli.
Amino Acid Sequence
MQLTVKALQG RECSLQVPED ELVSTLKQLV SEKLNVPVRQ QRLLFKGKAL ADGKRLSDYS IGPNSKLNLV VKPLEKVLLE EGEAQRLADS PPPQVWQLIS KVLARHFSAA DASRVLEQLQ RDYERSLSRL TLDDIERLAS RFLHPEVTET MEKGFSKLEH HHHHH.

Product Science Overview

Introduction

Ubiquitin-Like 4A (UBL4A), also known as GDX, is a protein that contains a ubiquitin-like domain. This protein plays a critical role in various cellular processes, including protein degradation and regulation of essential cellular functions. UBL4A is involved in post-translational modifications, which are crucial for maintaining cellular homeostasis.

Structure and Expression

UBL4A is a small protein with a molecular weight of approximately 18.8 kDa. It is composed of 157 amino acids and is often expressed with a C-terminal His-tag for purification purposes. The recombinant form of UBL4A is typically produced in Escherichia coli (E. coli) and purified using conventional chromatography techniques .

Biological Functions

UBL4A is involved in targeting cellular proteins for degradation. This process is essential for the regulation of protein levels within the cell and for the removal of damaged or misfolded proteins. In mammals, UBL4A plays a significant role during late spermiogenesis, a stage in sperm development where transcription is halted due to genome compaction. This creates a need for robust post-transcriptional regulation, which UBL4A helps to achieve .

Role in Cancer

Recent studies have highlighted the role of UBL4A in cancer, particularly in pancreatic ductal adenocarcinoma (PDAC). UBL4A has been found to inhibit autophagy-mediated proliferation and metastasis of cancer cells by targeting lysosome-associated membrane protein 1 (LAMP1). This interaction disrupts lysosomal function, leading to impaired autophagic degradation and accumulation of autophagosomes. High levels of UBL4A are associated with a favorable prognosis in PDAC patients, suggesting its potential as a therapeutic target .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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