UBE2T Human

Ubiquitin-Conjugating Enzyme E2T Human Recombinant
Cat. No.
BT19553
Source
Escherichia Coli.
Synonyms
EC 6.3.2.19, HSPC150, PIG50, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T, Ubiquitin carrier protein T, Cell proliferation-inducing gene 50 protein, UBE2T.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

UBE2T Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 205 amino acids (1-197 a.a.) and having a molecular mass of 23.6 kDa. The UBE2T is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
UBE2T, a member of the E2 ubiquitin-conjugating enzyme family, plays a crucial role in the protein degradation pathway. It facilitates the ATP-dependent attachment of ubiquitin to target proteins, marking them for destruction by the proteasome. This enzyme is essential for the Faconi anemia pathway, which is involved in DNA damage repair. Self-inactivation of UBE2T can negatively impact the Faconi pathway.
Description
Recombinant human UBE2T, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 205 amino acids (1-197 a.a.). It has a molecular weight of 23.6 kDa. This protein includes an 8 amino acid His Tag at the C-terminus and is purified through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized through filtration.
Formulation
The UBE2T solution is formulated with 20mM Tris-HCl at pH 8, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of UBE2T is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
EC 6.3.2.19, HSPC150, PIG50, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T, Ubiquitin carrier protein T, Cell proliferation-inducing gene 50 protein, UBE2T.
Source
Escherichia Coli.
Amino Acid Sequence
MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH.

Product Science Overview

Introduction

Ubiquitin-Conjugating Enzyme E2T (UBE2T), also known as HSPC150, is a member of the ubiquitin-conjugating enzyme family. These enzymes play a crucial role in the ubiquitination process, which tags proteins for degradation via the proteasome. UBE2T is involved in various cellular processes, including cell cycle regulation, DNA repair, and signal transduction.

Structure and Function

UBE2T contains a typical UBC domain, which allows it to interact with E3 ligases, such as the RING finger or HECT domain-containing proteins. This interaction facilitates the transfer of ubiquitin from the E2 enzyme to the substrate protein, leading to its mono- or polyubiquitination . UBE2T was initially identified as a key player in the Fanconi anemia (FA) pathway, where it is involved in DNA repair .

Role in Cancer

UBE2T has been implicated in various types of cancer, acting as an oncogene. It promotes tumor growth and progression by regulating the ubiquitination and degradation of tumor suppressor proteins. For instance, UBE2T has been shown to interact with the BRCA1/BARD1 complex, leading to the ubiquitination and downregulation of BRCA1 in breast cancer cells . This interaction is critical for the development and progression of breast cancer.

In lung adenocarcinoma, UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway . Overexpression of UBE2T has been associated with increased proliferation and autophagy in lung cancer cells, contributing to drug resistance and poor prognosis .

Clinical Significance

The clinical relevance of UBE2T extends beyond its role in cancer. Its expression levels and activity can serve as potential biomarkers for cancer diagnosis and prognosis. Additionally, targeting UBE2T and its associated pathways may offer new therapeutic strategies for cancer treatment.

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