UBE2S Antibody
Description
Product Specs
Product Science Overview
Ubiquitin Conjugating Enzyme E2S (UBE2S) is a crucial component of the ubiquitin-proteasome system (UPS), which is responsible for the targeted degradation of proteins within the cell. This system plays a vital role in maintaining cellular homeostasis by regulating the levels of various proteins, thus ensuring proper cellular function and response to environmental changes.
UBE2S is an E2 enzyme, which means it is involved in the second step of the ubiquitination process. The ubiquitination process involves three main types of enzymes:
- E1 (Ubiquitin-activating enzymes): These enzymes activate ubiquitin in an ATP-dependent manner.
- E2 (Ubiquitin-conjugating enzymes): These enzymes, including UBE2S, transfer the activated ubiquitin from the E1 enzyme to the substrate protein.
- E3 (Ubiquitin ligases): These enzymes facilitate the transfer of ubiquitin from the E2 enzyme to the target protein, thus tagging it for degradation by the proteasome.
UBE2S is known for its role in elongating ubiquitin chains on substrate proteins, a process that is essential for the recognition and degradation of these proteins by the proteasome. Specifically, UBE2S catalyzes the formation of K11-linked polyubiquitin chains, which are crucial for cell cycle regulation and mitosis .
UBE2S has been implicated in various cellular processes, including:
- Cell Cycle Regulation: UBE2S is essential for the proper progression of the cell cycle, particularly during mitosis. It ensures the timely degradation of cell cycle regulators, thus preventing abnormal cell division and proliferation .
- DNA Damage Response: UBE2S plays a role in the cellular response to DNA damage by regulating the stability of proteins involved in DNA repair mechanisms .
- Cancer: Dysregulation of UBE2S has been linked to the development and progression of various cancers. Overexpression of UBE2S has been observed in several types of cancer, including breast, lung, and colorectal cancers, where it contributes to uncontrolled cell proliferation and tumor growth .
The mouse anti-human UBE2S antibody is a monoclonal antibody specifically designed to recognize and bind to the human UBE2S protein. This antibody is commonly used in research to study the expression, localization, and function of UBE2S in various biological contexts. It is also utilized in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect UBE2S in cell and tissue samples .
